Farkas T, Tanaka A, Kai K, Kanoe M
Department of Veterinary Microbiology, Faculty of Agriculture, Yamaguchi University, Japan.
J Vet Med Sci. 1996 Jul;58(7):681-4. doi: 10.1292/jvms.58.681.
The complete genomic sequence of chicken anaemia virus (CAV) TK-5803 strain was determined. Comparisons of sequence data showed 37 nucleotide differences between TK-5803 and Cux-1, 38 nucleotide differences between TK-5803 and 26P4, and 48 nucleotide differences between TK-5803 and 82-2. There were 65 nucleotide differences in the largest open reading frame (ORF3) between TK-5803 and one Australian isolate. Base changes introduced amino acid changes at 6 positions in the C-terminal half of VP3, at 4 positions in the C-terminal quarter of VP2 and at 17 positions in VP1 among the strains. These indicate that the N-terminal half of VP3 and the N-terminal three quarter of VP2 are well conserved, and might sustain essential function of these proteins. The amino acid changes in VP1 which is thought to be the capsid protein may influence the antigenic character of different VP1s.
测定了鸡贫血病毒（CAV）TK - 5803株的全基因组序列。序列数据比较显示，TK - 5803与Cux - 1之间有37个核苷酸差异，TK - 5803与26P4之间有38个核苷酸差异，TK - 5803与82 - 2之间有48个核苷酸差异。TK - 5803与一株澳大利亚分离株之间在最大开放阅读框（ORF3）中有65个核苷酸差异。碱基变化在各毒株的VP3 C端一半的6个位置、VP2 C端四分之一的4个位置以及VP1的17个位置引入了氨基酸变化。这些表明VP3的N端一半和VP2的N端四分之三高度保守，可能维持这些蛋白质的基本功能。被认为是衣壳蛋白的VP1中的氨基酸变化可能会影响不同VP1的抗原特性。
