Håkansson A, Gustafsson B, Krysander L, Håkansson L
Department of Oncology, University Hospital, Linköping, Sweden.
Br J Cancer. 1996 Sep;74(5):670-6. doi: 10.1038/bjc.1996.420.
Interferon alpha (IFN-alpha) has a documented activity against malignant melanoma with a response rate of only approximately 20%. It would therefore be of considerable importance if patients likely to respond could be identified. The degree of mononuclear cell infiltration in primary tumours has been reported to correlate with a favourable prognosis. This investigation used monoclonal antibodies, anti-CD4, -CD8 and -CD11c, to identify subsets of tumour-infiltrating mononuclear cells in fine needle aspirates to study whether the presence of such cells correlates with the therapeutic effect of IFN-alpha. Twenty-one patients with systemic and 20 with regional metastatic malignant melanoma were studied before initiation of IFN-alpha treatment. A statistically significant correlation (P < 0.001) was found between the occurrence of CD4+ lymphocytes in fine needle aspirates and the therapeutic benefit of IFN-alpha in patients with systemic disease. Ten out of 11 with moderate to high numbers of infiltrating CD4+ lymphocytes achieved tumour regression. In contrast, among patients with low numbers of these cells in metastatic lesions, nine out of ten had progressive disease. Similar results were found in patients with regional disease.
α干扰素（IFN-α）对恶性黑色素瘤具有已被证实的活性，但其缓解率仅约为20%。因此，如果能够识别出可能有反应的患者，将具有相当重要的意义。据报道，原发性肿瘤中单核细胞浸润程度与预后良好相关。本研究使用抗CD4、抗CD8和抗CD11c单克隆抗体，在细针穿刺抽吸物中识别肿瘤浸润单核细胞亚群，以研究这些细胞的存在是否与IFN-α的治疗效果相关。在开始IFN-α治疗前，对21例全身性和20例区域性转移性恶性黑色素瘤患者进行了研究。在细针穿刺抽吸物中CD4+淋巴细胞的出现与全身性疾病患者IFN-α的治疗益处之间发现了具有统计学意义的相关性（P < 0.001）。11例有中度至高数量浸润性CD4+淋巴细胞的患者中有10例实现了肿瘤消退。相比之下，在转移病灶中这些细胞数量较少的患者中，10例中有9例病情进展。在区域性疾病患者中也发现了类似结果。
