在尸检脑组织中检测麻疹病毒核蛋白mRNA

Detection of measles virus nucleoprotein mRNA in autopsied brain tissues.

作者信息

Katayama Y, Hotta H, Nishimura A, Tatsuno Y, Homma M

机构信息

Department of Microbiology, Kobe University School of Medicine, Hyogo, Japan.

出版信息

J Gen Virol. 1995 Dec;76 ( Pt 12):3201-4. doi: 10.1099/0022-1317-76-12-3201.

DOI:10.1099/0022-1317-76-12-3201

PMID:8847530

Abstract

By means of RT-PCR, a portion of measles virus (MV) mRNA encoding nucleoprotein (NP) could be detected in 11 (18%) of 61 brain tissue samples obtained from administrative autopsy cases, who apparently had not suffered from subacute sclerosing panencephalitis (SSPE)-like central nervous system disorders. Most of the brain-derived NP sequences showed significant asynonymous nucleotide substitutions when compared with wild-type MV isolates and SSPE virus. Our present results suggest that MV commonly persists in the human brain without causing apparent clinical symptoms, probably due to decreased virus replication.

摘要

通过逆转录聚合酶链反应（RT-PCR），在从行政尸检病例获得的61份脑组织样本中的11份（18%）中检测到了一部分编码核蛋白（NP）的麻疹病毒（MV）mRNA，这些病例显然未患有亚急性硬化性全脑炎（SSPE）样中枢神经系统疾病。与野生型MV分离株和SSPE病毒相比，大多数源自脑的NP序列显示出明显的非同义核苷酸替换。我们目前的结果表明，MV通常在人脑中持续存在而不引起明显的临床症状，这可能是由于病毒复制减少所致。