Oliveira M I, Curti S P, Figueiredo C A, Sardinha A M A, Sallum M A M, Durigon E L
Instituto Adolfo Lutz, São Paulo, Brasil.
Acta Virol. 2004;48(1):9-14.
This study reports on molecular analysis of a Measles virus (MV) isolate from a patient who was infected in Japan but showed symptoms after arriving to Brazil. This patient had typical clinical measles infection symptoms: fever, rash, cough and coryza. After isolating the virus in B95a cells, a fragment of the nucleoprotein (N) gene was amplified by reverse transcription-polymerase chain reaction (RT-PCR) and subjected to direct nucleotide sequencing. The sequence data showed that the MV isolate of concern is of the D5 genotype.
本研究报告了对一名在日本感染但抵达巴西后出现症状的患者的麻疹病毒（MV）分离株的分子分析。该患者有典型的临床麻疹感染症状：发热、皮疹、咳嗽和鼻炎。在B95a细胞中分离出病毒后，通过逆转录-聚合酶链反应（RT-PCR）扩增核蛋白（N）基因的一个片段，并进行直接核苷酸测序。序列数据显示，所关注的MV分离株属于D5基因型。
