Potworowski E F, Gagnon F, Beauchemin C, St Pierre Y
Centre de Recherche en Immunologie, Institut Armand-Frappier, Laval-des Rapides, QC, Canada.
Leukemia. 1996 Oct;10(10):1639-47.
Thymic lymphomas develop in C57BL/Ka mice within 36 weeks after split-dose X-irradiation. Lymphoma development can be abrogated in such mice by the injection of syngeneic bone marrow from healthy donors. The abrogation mechanism is unknown, but since bone marrow supplies the thymus with precursors of thymocytes and of dendritic cells, we tested the ability of early thymocytes and of immortalized thymic dendritic cells to abrogate lymphomagenesis. Fifteen weeks after irradiation, mice which had received bone marrow or dendritic cells had an equally low incidence of lymphoma, whereas mice which had received thymocytes or which had been only irradiated developed equally high levels of lymphomas, indicating that thymic dendritic cells played a key role in the prevention of lymphoma development. When thymuses from 15-week survivors were tested for pre-lymphoma cells, those from dendritic cell-treated mice proved to be endowed with a level of lymphomagenic potential intermediate between that from bone marrow-treated mice (nonlymphomagenic) and that from untreated or thymocyte-treated mice (highly lymphomagenic). These data indicate that lymphoma abrogation by bone marrow cells involves the participation of marrow-derived thymic dendritic cells.
胸腺淋巴瘤在经分次剂量X射线照射后的C57BL/Ka小鼠中，36周内发生。通过注射来自健康供体的同基因骨髓，可消除此类小鼠中的淋巴瘤发生。消除机制尚不清楚，但由于骨髓为胸腺提供胸腺细胞和树突状细胞的前体，我们测试了早期胸腺细胞和永生化胸腺树突状细胞消除淋巴瘤发生的能力。照射后15周，接受骨髓或树突状细胞的小鼠淋巴瘤发病率同样低，而接受胸腺细胞或仅接受照射的小鼠淋巴瘤发生率同样高，这表明胸腺树突状细胞在预防淋巴瘤发生中起关键作用。当对15周存活小鼠的胸腺进行淋巴瘤前体细胞检测时，来自树突状细胞处理小鼠的胸腺具有的淋巴瘤发生潜能水平介于来自骨髓处理小鼠（无淋巴瘤发生潜能）和未处理或胸腺细胞处理小鼠（高淋巴瘤发生潜能）之间。这些数据表明，骨髓细胞消除淋巴瘤涉及骨髓来源的胸腺树突状细胞的参与。
