Pereira B, Rosa L F, Safi D A, Bechara E J, Curi R
Department of Biochemistry, Universidade de São Paulo, Brazil.
Biochem Pharmacol. 1995 Dec 22;50(12):2093-8. doi: 10.1016/0006-2952(95)02116-7.
This study examined the effects of glycocorticoids, insulin, thyroxine, and epinephrine upon the activities of CuZn- and Mn-superoxide dismutases (SOD), catalase, and glutathione peroxidase (GPX) and upon hydrogen peroxide production in rat macrophages obtained from the intraperitoneal cavity. The experiments were performed in vivo under conditions causing hormonal dysfunctions: adrenal demedullation, dexamethasone treatment, thyroidectomy, administration of L-tri-iodothyronine (T3) and L-thyroxine (T4), and diabetes. Macrophages were also cultured for 24 hr in the presence of dexamethasone, thyroid hormones, and insulin as to evaluate possible interferences caused in vivo by changes in other hormones. The results indicated that these hormones do control the activities of the antioxidant enzymes and hydrogen peroxide production both in vivo and in vitro. Insulin increased the activities of CuZn-SOD, catalase, and GPX and reduced that of Mn-SOD. Thyroid hormones raised the activities of CuZn- and Mn-SOD and decreased that of GPX, whereas glucocorticoids reduced both Mn-SOD and GPX. The removal of the adrenal medulla caused a decrease of Mn-SOD and GPX activities in the macrophages. Hydrogen peroxide production was increased by insulin and reduced by thyroid hormones and glucocorticoids. The changes in antioxidant enzyme activities caused by these hormones in macrophages may indicate important mechanisms for the establishment of impaired immune function in endocrine pathologies.
本研究检测了糖皮质激素、胰岛素、甲状腺素和肾上腺素对从大鼠腹腔获取的巨噬细胞中铜锌超氧化物歧化酶（CuZn-SOD）、锰超氧化物歧化酶（Mn-SOD）、过氧化氢酶和谷胱甘肽过氧化物酶（GPX）活性以及过氧化氢生成的影响。实验在导致激素功能紊乱的体内条件下进行：肾上腺髓质切除、地塞米松治疗、甲状腺切除、给予左旋三碘甲状腺原氨酸（T3）和左旋甲状腺素（T4）以及糖尿病。巨噬细胞还在存在地塞米松、甲状腺激素和胰岛素的情况下培养24小时，以评估体内其他激素变化可能引起的干扰。结果表明，这些激素在体内和体外均能控制抗氧化酶的活性和过氧化氢的生成。胰岛素增加了CuZn-SOD、过氧化氢酶和GPX的活性，降低了Mn-SOD的活性。甲状腺激素提高了CuZn-SOD和Mn-SOD的活性，降低了GPX的活性，而糖皮质激素则降低了Mn-SOD和GPX的活性。肾上腺髓质切除导致巨噬细胞中Mn-SOD和GPX活性降低。胰岛素增加过氧化氢生成，甲状腺激素和糖皮质激素则降低过氧化氢生成。这些激素引起的巨噬细胞抗氧化酶活性变化可能表明在内分泌疾病中建立免疫功能受损的重要机制。
