Khan S R, Atmani F, Glenton P, Hou Z, Talham D R, Khurshid M
Department of Pathology, Box 100275, College of Medicine, University of Florida, Gainesville, Florida 32610, USA.
Calcif Tissue Int. 1996 Nov;59(5):357-65. doi: 10.1007/s002239900140.
The ultrastructure of the organic matrix of demineralized urinary stones was examined by standard transmission and scanning electron microscopy as well as after malachite green-glutaraldehyde fixation. Crystal ghosts of both calcium oxalate and calcium phosphate were made of amorphous material and were dispersed in a matrix containing amorphous, fibrillar, and membranous substances. Malachite green positive material was seen to be associated with the ghosts, as well as with the membranous and fibrillar components of the organic matrix. Calcium oxalate and calcium-phosphate crystals, induced in human urine in vitro were also found to be associated with an organic matrix containing lipids and proteins. It is suggested that the intimate association between crystals and lipids is a result of the involvement of cellular membranes in the nucleation of these crystals.
通过标准透射电子显微镜和扫描电子显微镜以及孔雀石绿-戊二醛固定后，对脱矿化尿结石有机基质的超微结构进行了检查。草酸钙和磷酸钙的晶体幽灵均由无定形物质构成，并分散在含有无定形、纤维状和膜状物质的基质中。可见孔雀石绿阳性物质与晶体幽灵以及有机基质的膜状和纤维状成分相关。体外在人尿液中诱导形成的草酸钙和磷酸钙晶体也被发现与含有脂质和蛋白质的有机基质相关。有人提出，晶体与脂质之间的紧密关联是细胞膜参与这些晶体成核的结果。
