Broll R, Düren G, Gründig I, Eggers R, Busch L, Herold A, Schiedeck T, Bruch H P
Klinik für Chirurgie, Medizinischen Universität zu Lübeck.
Chirurg. 1996 Jan;67(1):53-8.
In this study we expanded ileal segments of 8 pigs with an intraluminal expander over a period of 6 to 8 weeks till the volume had reached approximately sixfold of the starting volume. The morphological and morphometrical examinations showed flattened villi with elongated crypts and especially an increase in the thickness of the muscularis. The villi appeared flattened through scanning microscopy as well, but the structure of the microvilli was nearly normal. The proliferative activity in the muscularis, estimated immunohistochemically by a monoclonal antibody against PCNA, showed a threefold increase. So it can be assumed, that the increased thickness of the muscle layer was caused not only by muscle cell hypertrophy but also by hyperplasia. The activity of the alpha-glucosidase, a brush border enzyme, was clearly reduced. The minor cause of this effect was the direct damage of the microvilli from the expander, but the major cause was the exclusion of the ileal segment from the small bowel passage and the resulting lack of nutrients. With our technique it seems possible to create an ileum pouch, a stomach substitute or a urinary bladder substitute.
在本研究中，我们使用腔内扩张器对8头猪的回肠段进行了6至8周的扩张，直至其体积达到起始体积的约6倍。形态学和形态计量学检查显示，绒毛变平，隐窝拉长，尤其是肌层厚度增加。通过扫描显微镜观察，绒毛也呈现扁平状，但微绒毛结构基本正常。用抗增殖细胞核抗原的单克隆抗体进行免疫组织化学评估，肌层中的增殖活性增加了两倍。因此，可以推测，肌层厚度增加不仅是由于肌肉细胞肥大，还由于增生。刷状缘酶α-葡萄糖苷酶的活性明显降低。造成这种影响的次要原因是扩张器对微绒毛的直接损伤，但主要原因是回肠段被排除在小肠通道之外，导致营养物质缺乏。用我们的技术似乎有可能制造出回肠袋、胃替代物或膀胱替代物。
