Atkins G J, Sheahan B J, Liljeström P
Department of Microbiology, Trinity College, Dublin, Ireland.
Mol Biotechnol. 1996 Feb;5(1):33-8. doi: 10.1007/BF02762410.
The Semliki Forest virus (SFV) expression vector consists of a plasmid based on the SFV infectious clone. Foreign genes may be inserted into the structural coding region, transcribed as RNA, and expressed in cell culture after transfection. RNA containing inserted sequences may be packaged into virions using a helper systems. This allows efficient infection and expression without chemical transfection, but only one round of multiplication is possible. The biosafety of the system has been increased by the introduction of multiple mutations, specifying a maturation defect, into the helper. Potential vaccines can be constructed by insertion of genes coding for antigenic proteins into the vector. Following insertion of the influenza virus nucleoprotein (NP) into the SFV vector, immunity was induced following injection of packaged or naked RNA into mice. The SFV vector is a "suicide" expression vector that has great potential for the construction of vaccines for both human and veterinary use.
Semliki森林病毒（SFV）表达载体由基于SFV感染性克隆的质粒组成。外源基因可插入结构编码区，转录为RNA，并在转染后在细胞培养物中表达。含有插入序列的RNA可使用辅助系统包装成病毒粒子。这允许在不进行化学转染的情况下进行高效感染和表达，但仅可能进行一轮增殖。通过在辅助病毒中引入多个导致成熟缺陷的突变，提高了该系统的生物安全性。通过将编码抗原蛋白的基因插入载体中，可以构建潜在的疫苗。将流感病毒核蛋白（NP）插入SFV载体后，将包装好的或裸露的RNA注射到小鼠体内可诱导免疫反应。SFV载体是一种“自杀”表达载体，在构建人用和兽用疫苗方面具有巨大潜力。
