Catapano G, Di Lorenzo M C, Della Volpe C, De Bartolo L, Migliaresi C
Department of Chemical and Materials Engineering, University of Calabria, Arcavacata di Rende (CS), Italy.
J Biomater Sci Polym Ed. 1996;7(11):1017-27. doi: 10.1163/156856296x00417.
Extracorporeal therapies based on membrane hybrid liver support devices using primary hepatocytes are an interesting approach to the treatment of acute hepatic failure. In such devices, semipermeable polymeric membranes are effectively used as immunoselective barriers between a patient's blood and the xenocytes in order to prevent the immune rejection of the graft. The membranes may act also as the substratum for cell adhesion, thus favouring the viability and functions of anchorage-dependent cells such as the hepatocytes. Membrane cytocompatibility is expected to depend on the surface properties of the polymer, such as its morphology and its physico-chemical properties. In this paper, we report our investigation on the effect of the surface wettability of membranes on hepatocyte viability and functions. Polypropylene microporous membranes were modified to increase their surface wettability and were used as substrata for rat hepatocyte adhesion culture. Isolated hepatocytes were also cultured on collagen as a reference substratum. Hepatocyte viability generally improved as the cells were cultured on more wettable membranes. In agreement with the viability data, the increasing wettability of the membrane surface also improved some metabolic functions.
基于使用原代肝细胞的膜混合肝支持装置的体外治疗方法是治疗急性肝衰竭的一种有趣途径。在这类装置中，半透性聚合物膜被有效地用作患者血液与异种细胞之间的免疫选择性屏障，以防止移植物的免疫排斥反应。这些膜还可作为细胞黏附的基质，从而有利于诸如肝细胞等锚定依赖性细胞的存活和功能。膜的细胞相容性预计取决于聚合物的表面性质，例如其形态和物理化学性质。在本文中，我们报告了关于膜表面润湿性对肝细胞活力和功能影响的研究。对聚丙烯微孔膜进行改性以提高其表面润湿性，并将其用作大鼠肝细胞黏附培养的基质。分离的肝细胞也在胶原蛋白上作为参考基质进行培养。随着细胞在更具润湿性的膜上培养，肝细胞活力总体上有所提高。与活力数据一致，膜表面润湿性的增加也改善了一些代谢功能。
