Crossman M W, Hirschberg C B
J Biol Chem. 1977 Aug 25;252(16):5815-9.
Labeled C18-phytosphingosine was detected in a glyco-lipid-rich fraction from rat intestine 4 and 24 h after injection of D-erythro[4,5-3H]C18-dihydrosphingosine. The criteria for identification were: (a) co-migration on gas-liquid chromatography of the trimethylsilyl ethers of the free and N-acetylated base with authentic standard, (b) co-migration on thin layer chromatography of the dinitrophenyl derivative with authentic standard, (c) labeled pentadecanol detection by gas-liquid radiochromatography after subjecting intact sphingolipids or a mixture of free long chain bases to periodate oxidation followed by sodium borohydride reduction, and (d) obtainment of the same fragmentation pattern as with authentic standard when the trimethylsilyl ethers of the N-acetylated base were subjected to gas-liquid chromatography-mass spectrometry. Smaller amounts of labeled C18-phytosphingosine were also detected in ceramide- and sphingomyelin-rich fractions of intestine and in liver and kidney. Experiments with germ-free rats strongly suggest that the intestinal flora is not the (sole) site for the biosynthesis of C18-phytosphingosine.
注射D-赤藓糖型[4,5-³H]C18-二氢鞘氨醇后4小时和24小时，在大鼠肠道富含糖脂的部分检测到标记的C18-植物鞘氨醇。鉴定标准如下：(a) 游离碱和N-乙酰化碱的三甲基硅醚在气液色谱上与标准品共迁移；(b) 二硝基苯基衍生物在薄层色谱上与标准品共迁移；(c) 完整鞘脂或游离长链碱混合物经高碘酸盐氧化后用硼氢化钠还原，通过气液放射性色谱检测标记的十五烷醇；(d) N-乙酰化碱的三甲基硅醚进行气液色谱-质谱分析时，获得与标准品相同的碎片模式。在肠道富含神经酰胺和鞘磷脂的部分以及肝脏和肾脏中也检测到少量标记的C18-植物鞘氨醇。无菌大鼠实验强烈表明，肠道菌群不是C18-植物鞘氨醇生物合成的（唯一）场所。
