Bioseparation Technology Laboratory, Biochemistry and Biophysics Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892-1762, USA.
J Sep Sci. 2011 May;34(10):1205-11. doi: 10.1002/jssc.201100019. Epub 2011 Apr 13.
Three types of novel coiled column configurations, i.e. a triangular coiled column and elliptical coiled columns I and II, were designed for type-I countercurrent chromatography and their performances were evaluated with two solvent systems each with suitable test samples. Three dinitrophenyl (DNP) amino acids (DNP-DL-glu, DNP-β-ala and DNP-L-ala) were separated with a moderately hydrophobic two-phase solvent system composed of hexane-ethyl acetate-metanol-0.1 M hydrochloric acid (1:1:1:1, v/v), while two dipeptides (tryptophyl-tyrosine and valyl-tyrosine) were separated with a polar solvent system composed of 1-butanol-acetic acid-water (4.75:0.25:5, v/v). The overall results indicated that the performance of compact type-I countercurrent chromatography was improved by elliptical coiled column I which was mounted with its maximum coil diameter perpendicular to the surface of the column holder. Hydrodynamic effects involved in these separations were discussed.
设计了三种新型螺旋柱构型，即三角形螺旋柱和椭圆形螺旋柱 I 和 II，用于第一类逆流色谱，并使用两种合适的测试样品的溶剂系统对其性能进行了评估。使用由正己烷-乙酸乙酯-甲醇-0.1 M 盐酸（1:1:1:1，v/v）组成的中等疏水性两相溶剂系统分离了三种二硝基苯（DNP）氨基酸（DNP-DL-glu、DNP-β-ala 和 DNP-L-ala），而使用由 1-丁醇-乙酸-水（4.75:0.25:5，v/v）组成的极性溶剂系统分离了两种二肽（色氨酰-酪氨酸和缬氨酰-酪氨酸）。总体结果表明，通过将最大螺旋直径垂直于柱架表面安装的椭圆形螺旋柱 I，紧凑型第一类逆流色谱的性能得到了改善。讨论了这些分离中涉及的流体动力学效应。
