Bell J G, Ashton I, Secombes C J, Weitzel B R, Dick J R, Sargent J R
N.E.R.C. Unit of Aquatic Biochemistry, School of Natural Sciences, University of Stirling, Stirling, UK.
Prostaglandins Leukot Essent Fatty Acids. 1996 Mar;54(3):173-82. doi: 10.1016/s0952-3278(96)90013-7.
Atlantic salmon (Salmo salar) post-smolts were fed diets containing either Fosol (FO), a North Sea fish oil, sunflower oil (SO), linseed oil (LO) or Marinol K (MO), a southern hemisphere fish oil rich in 20:5(n-3) for 12 weeks. A macrophage-enriched leucocyte preparation was obtained from head kidney and the fatty acid compositions of the individual membrane phospholipids measured. In general phospholipids from SO- and LO-fed fish had increased 18:2(n-6), 20:2(n-6) and 20:3(n-6) compared to the fish oil treatments while LO-fed fish had lower 20:4(n-6) than any other dietary treatment. Fish fed LO also had increased 18:3(n-3), 18:4(n-3), 20:3(n-3) and 20:4(n-3). The 20:5(n-3) content of kidney macrophage-enriched leucocyte phospholipids was highest in MO-fed fish followed by FO- and LO-fed fish with the lowest level in fish fed SO. The overall effect on the ratio of eicosanoid precursors, 20:4/20:5, showed the highest value in SO-fed fish and the lowest in fish fed LO. Production of LTB5 by kidney macrophage-enriched leucocytes stimulated with A23187 was highest in MO-fed fish and lowest in those fed SO. Production of LTB4 was greatest in SO-fed fish and lowest in fish fed LO. Serum Ig levels were significantly affected by dietary treatment with highest values in fish fed FO and SO and lowest in fish fed MO and LO.
将大西洋鲑鱼（Salmo salar）的后洄游幼鱼投喂含有以下成分的饲料12周：Fosol（FO），一种北海鱼油；葵花籽油（SO）；亚麻籽油（LO）；或Marinol K（MO），一种富含20:5(n-3)的南半球鱼油。从头部肾脏获得富含巨噬细胞的白细胞制剂，并测量各个膜磷脂的脂肪酸组成。总体而言，与鱼油处理组相比，饲喂SO和LO的鱼的磷脂中18:2(n-6)、20:2(n-6)和20:3(n-6)含量增加，而饲喂LO的鱼的20:4(n-6)含量低于任何其他饮食处理组。饲喂LO的鱼的18:3(n-3)、18:4(n-3)、20:3(n-3)和20:4(n-3)含量也有所增加。肾脏富含巨噬细胞的白细胞磷脂中的20:5(n-3)含量在饲喂MO的鱼中最高，其次是饲喂FO和LO的鱼，饲喂SO的鱼中含量最低。对类二十烷酸前体比例20:4/20:5的总体影响显示，饲喂SO的鱼中该比例最高，饲喂LO的鱼中最低。用A23187刺激肾脏富含巨噬细胞的白细胞产生LTB5的量在饲喂MO的鱼中最高，在饲喂SO的鱼中最低。LTB4的产生在饲喂SO的鱼中最大，在饲喂LO的鱼中最小。血清Ig水平受饮食处理的显著影响，饲喂FO和SO的鱼中血清Ig水平最高，饲喂MO和LO的鱼中最低。
