Ras N M, Lascola B, Postic D, Cutler S J, Rodhain F, Baranton G, Raoult D
Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, Paris, France.
Int J Syst Bacteriol. 1996 Oct;46(4):859-65. doi: 10.1099/00207713-46-4-859.
The phylogenetic relationships of 20 relapsing fever (RF) Borrelia spp. were estimated on the basis of the sequences of rrs genes. Complete sequences were aligned and compared with previously published sequences, and the similarity values were found to be 97.7 to 99.9%. Phylogenetic trees were constructed by using the three neighbor-joining, maximum-parsimony, and maximum-likelihood methods. The results of the comparative phylogenetic analysis divided the RF Borrelia spp. into three major clusters. One cluster included Borrelia crocidurae, Borrelia duttonii, Borrelia recurrentis, and Borrelia hispanica. Another cluster comprised tow main branches with Borrelia coriaceae, Borrelia lonestari, and Borrelia miyamotoi on one side and Borrelia parkeri, Borrelia turicatae, and Borrelia hermsii on the other side. Borrelia anserina constituted the third cluster. The phylogenetic position of Borrelia persica was more uncertain. These results suggested that the taxonomy of these spirochetes should be revised. To overcome the problems of culturing the spirochetes, RF Borrelia primers were defined. Following PCR amplification of the rrs gene, restriction length fragment polymorphism could be used to distinguish between RF Borrelia strains.
基于16S rRNA（rrs）基因序列，对20种复发性发热（RF）疏螺旋体物种的系统发育关系进行了评估。将完整序列进行比对，并与先前发表的序列进行比较，发现相似性值为97.7%至99.9%。使用邻接法、最大简约法和最大似然法构建了系统发育树。比较系统发育分析的结果将RF疏螺旋体物种分为三个主要类群。一个类群包括黄头鼠疏螺旋体、杜氏疏螺旋体、回归热疏螺旋体和西班牙疏螺旋体。另一个类群由两个主要分支组成，一侧是科氏疏螺旋体、洛氏疏螺旋体和宫本疏螺旋体，另一侧是帕克疏螺旋体、蜱传疏螺旋体和赫氏疏螺旋体。鹅疏螺旋体构成第三个类群。波斯疏螺旋体的系统发育位置更不确定。这些结果表明，这些螺旋体的分类应予以修订。为克服螺旋体培养的问题，确定了RF疏螺旋体引物。在对rrs基因进行PCR扩增后，限制性片段长度多态性可用于区分RF疏螺旋体菌株。
