Department of Molecular Genetics and Microbiology, Center for Infectious Diseases, Stony Brook University, Stony Brook, NY 11794-5120, USA.
J Clin Microbiol. 2010 Jul;48(7):2484-9. doi: 10.1128/JCM.00541-10. Epub 2010 May 12.
Multilocus sequence analysis (MLSA) was used to clarify the taxonomic status of a virulent Borrelia organism previously isolated from patients with relapsing fever and from ticks in Spain that is designated the Spanish relapsing fever (SRF) Borrelia. This species has been used extensively in experimental infection models because of its continued virulence. Seven genes were amplified to analyze the phylogenetic relationships among several Spanish isolates of SRF Borrelia and other relapsing fever Borrelia species. The genes targeted in this study included rrs and flaB, which have commonly been used in phylogenetic studies; the rrf-rrl intergenic spacer (IGS), which is highly discriminatory; and four additional genes, p66, groEL, glpQ, and recC, which are located on the chromosome and which have therefore evolved in a clonal way. The species included in this study were Borrelia duttonii, B. recurrentis, B. crocidurae, and B. hispanica as Old World Borrelia species and B. turicatae and B. hermsii as New World Borrelia species. The results obtained by MLSA of the SRF Borrelia on the basis of 1% of the genomic sequence data analyzed confirmed that the SRF Borrelia isolates are B. hispanica. However, the prototype isolates of B. hispanica used in this study have an uncertain history and display unique phenotypic characteristics that are not shared with the SRF Borrelia. Therefore, we propose to use strain SP1, isolated from a relapsing fever patient in 1994 in southern Spain, as the type strain for B. hispanica.
多位点序列分析（MLSA）被用于阐明先前从西班牙复发热患者和蜱中分离出的一种具有毒性的伯氏疏螺旋体的分类地位，该螺旋体被指定为西班牙复发热（SRF）伯氏疏螺旋体。由于其持续的毒性，该物种已被广泛用于实验感染模型。本研究中扩增了 7 个基因，以分析几种西班牙分离的 SRF 伯氏疏螺旋体和其他复发热伯氏疏螺旋体物种的系统发育关系。本研究中靶向的基因包括 rrs 和 flaB，它们通常用于系统发育研究；rrf-rrl 基因间间隔区（IGS），其具有高度区分性；以及另外 4 个基因 p66、groEL、glpQ 和 recC，它们位于染色体上，因此以克隆方式进化。本研究中包括的物种为旧世界伯氏疏螺旋体物种的 B. duttonii、B. recurrentis、B. crocidurae 和 B. hispanica，以及新世界伯氏疏螺旋体物种的 B. turicatae 和 B. hermsii。根据分析的基因组序列数据的 1%进行的 SRF 伯氏疏螺旋体的 MLSA 结果证实，SRF 伯氏疏螺旋体分离株是 B. hispanica。然而，本研究中使用的 B. hispanica 原型分离株的历史不确定，并且表现出与 SRF 伯氏疏螺旋体不同的独特表型特征。因此，我们建议使用 1994 年在西班牙南部从复发热患者中分离出的 SP1 菌株作为 B. hispanica 的模式株。
