Mullany P, Pallen M, Wilks M, Stephen J R, Tabaqchali S
Department of Molecular and Cell Biology, Marischal College, University of Aberdeen, UK.
Gene. 1996 Sep 26;174(1):145-50. doi: 10.1016/0378-1119(96)00511-2.
We have been studying the conjugative transposon Tn5397, originally isolated from the Gram-positive pathogen Clostridium difficile. Physical analysis of this transposon demonstrated that it contained a group II intron. This is the first report of an intron in a conjugative transposon and the first report of a group II intron in Gram-positive bacteria. The intron interrupted a gene in Tn5397 that is almost identical to orf14 from Tn916. DNA hybridisation analysis showed that elements related to Tn5397, containing the group II intron, were present in five other C. difficile strains from different geographical locations suggesting that the element is likely to be widely distributed.
我们一直在研究接合转座子Tn5397，它最初是从革兰氏阳性病原体艰难梭菌中分离出来的。对该转座子的物理分析表明，它含有一个II组内含子。这是关于接合转座子中内含子的首次报道，也是革兰氏阳性细菌中II组内含子的首次报道。该内含子中断了Tn5397中一个与Tn916的orf14几乎相同的基因。DNA杂交分析表明，来自不同地理位置的其他5株艰难梭菌菌株中存在与含有II组内含子的Tn5397相关的元件，这表明该元件可能广泛分布。
