Bjerkli I H, Myklebust R, Räisänen S, Telimaa S, Stenfors L E
Department of Otolaryngology, University of Tromse, Norway.
Int J Pediatr Otorhinolaryngol. 1996 Aug;36(3):205-13. doi: 10.1016/0165-5876(96)01344-4.
On day 2 post partum, swab samples were obtained from the oropharyngx of 20 healthy, breast-fed babies. The samples were examined for aerobes (culture on agar plates), for bacterial coating with the immunoglobulins SIgA, IgG and IgM (immunofluorescence assay), for bacterial attachment to epithelial cells (fluorescence microscopy of acridine orange stained material, and scanning electron microscopy). alpha-Hemolytic streptococci grew almost exclusively in the oropharynx, while 32% (median value) of the epithelial cells showed bacterial attachment in abundance, viz. > 50 attached bacteria per cell, 66% (median value) of the bacteria showed positive reactivity when treated with anti-human SIgA serum. 72% (median value) of the bacteria were coated with IgG, but no IgM coating of the bacteria could be detected. Newborns do not possess IgA antibodies, as only IgG is able to pass the placental barrier. The SIgA-rich colostrum is capable of coating the oropharyngeal bacteria of breast-fed babies during their first days after birth. However, despite abundant SIgA-coating, bacteria still succeed in attaching to the epithelial cells of the oropharynx. This finding hints that factors other than SIgA impede the bacterial/cellular adhesion mechanism.
产后第2天，从20名健康的母乳喂养婴儿的口咽部采集拭子样本。对样本进行需氧菌检测（琼脂平板培养）、检测细菌表面的免疫球蛋白SIgA、IgG和IgM（免疫荧光测定）、检测细菌与上皮细胞的附着情况（吖啶橙染色材料的荧光显微镜检查和扫描电子显微镜检查）。α-溶血性链球菌几乎仅在口咽部生长，而32%（中位数）的上皮细胞显示有大量细菌附着，即每个细胞附着>50个细菌，66%（中位数）的细菌在用抗人SIgA血清处理时显示阳性反应。72%（中位数）的细菌被IgG包被，但未检测到细菌有IgM包被。新生儿不具备IgA抗体，因为只有IgG能够穿过胎盘屏障。富含SIgA的初乳能够在出生后的头几天包被母乳喂养婴儿口咽部的细菌。然而，尽管有大量的SIgA包被，细菌仍然成功附着在口咽部的上皮细胞上。这一发现提示，除SIgA外的其他因素阻碍了细菌/细胞黏附机制。
