Stenfors L E, Räisänen S
Department of Otolaryngology, University of Tromsö, Norway.
Acta Otolaryngol. 1991;111(6):1139-45. doi: 10.3109/00016489109100769.
Bacterial samples were obtained from the posterior wall of the nasopharynx (NPH) of 18 healthy children (age range 1 to 14 years, 10 males, 8 females) and subjected for immunofluorescence studies using fluorescein-labelled goat anti-human IgG and goat anti-human SIgA antibodies. Quantitative and qualitative bacteriological analyses were performed simultaneously. IgG-coated bacteria in the NPH were observed in 1-year-old subjects and opsonized bacteria seemed to increase in numbers and fluorescence intensity with increasing age. SIgA-coated bacteria appeared later, and intensely fluorescing bacteria were not seen until the age of 3 to 6 years. Thirteen out of the 18 children harboured middle ear pathogens in the NPH, but generally speaking non-pathogens dominated the bacterial flora. Immunoglobulins are of the utmost importance for the mucosal defence system, including protection against bacterial attachment to the epithelial cells (SIgA), and bacteriolysis and opsonization of the bacteria (IgG).
从18名健康儿童（年龄范围为1至14岁，10名男性，8名女性）的鼻咽后壁（NPH）获取细菌样本，并使用荧光素标记的山羊抗人IgG和山羊抗人SIgA抗体进行免疫荧光研究。同时进行了定量和定性细菌学分析。在1岁的受试者中观察到NPH中有IgG包被的细菌，并且随着年龄的增长，调理素化的细菌数量和荧光强度似乎增加。SIgA包被的细菌出现较晚，直到3至6岁才见到强荧光细菌。18名儿童中有13名在NPH中携带中耳病原体，但一般来说非病原体在细菌菌群中占主导地位。免疫球蛋白对于粘膜防御系统至关重要，包括防止细菌附着于上皮细胞（SIgA）以及细菌的溶菌作用和调理作用（IgG）。
