苏云金芽孢杆菌cryV型杀虫蛋白基因的克隆：cryV编码蛋白在稳定期早期表达。
Cloning of a cryV-type insecticidal protein gene from Bacillus thuringiensis: the cryV-encoded protein is expressed early in stationary phase.
作者信息
Kostichka K, Warren G W, Mullins M, Mullins A D, Palekar N V, Craig J A, Koziel M G, Estruch J J
机构信息
Insect Control--Seeds, CIBA Agricultural Biotechnology, Research Triangle Park, Durham, North Carolina 27709, USA.
出版信息
J Bacteriol. 1996 Apr;178(7):2141-4. doi: 10.1128/jb.178.7.2141-2144.1996.
Abstract
A CryV-type protein (CGCryV) has been isolated from supernatant fluids of Bacillus thuringiensis AB88 cultures. Previous reports have suggested the cryptic nature of the cryV-type genes on the basis of the absence of CryV-type proteins in parasporal crystals. The CryV-type protein reported here is expressed early in stationary phase, and evidence indicates that it is an exported protein. Analysis of the deduced protein sequence from this gene reveals the presence of an N-terminal domain that likely acts as a signal peptide. The CGCryV protein is the first reported case of a delta-endotoxin being a secreted protein, which may influence the biological relevance of these proteins.
摘要
已从苏云金芽孢杆菌AB88培养物的上清液中分离出一种CryV型蛋白（CGCryV）。先前的报道基于伴孢晶体中不存在CryV型蛋白，推测cryV型基因具有隐匿性。此处报道的CryV型蛋白在稳定期早期表达，且有证据表明它是一种分泌蛋白。对该基因推导的蛋白质序列分析显示存在一个可能作为信号肽的N端结构域。CGCryV蛋白是首例被报道的δ-内毒素为分泌蛋白的情况，这可能会影响这些蛋白的生物学相关性。
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