苏云金芽孢杆菌中cryV型杀虫蛋白基因的分布以及从苏云金芽孢杆菌库斯塔克亚种和苏云金芽孢杆菌昆虫致病亚种中克隆cryV型基因
Distribution of cryV-type insecticidal protein genes in Bacillus thuringiensis and cloning of cryV-type genes from Bacillus thuringiensis subsp. kurstaki and Bacillus thuringiensis subsp. entomocidus.
作者信息
Shin B S, Park S H, Choi S K, Koo B T, Lee S T, Kim J I
机构信息
Genetic Engineering Research Institute, Korea Institute of Science and Technology, Taejon.
出版信息
Appl Environ Microbiol. 1995 Jun;61(6):2402-7. doi: 10.1128/aem.61.6.2402-2407.1995.
Abstract
DNA dot blot hybridizations with a cryV-specific probe and a cryI-specific probe were performed to screen 24 Bacillus thuringiensis strains for their cryV-type (lepidopteran- and coleopteran-specific) and cryI-type (lepidopteran-specific) insecticidal crystal protein gene contents, respectively. The cryV-specific probe hybridized to 12 of the B. thuringiensis strains examined. Most of the cryV-positive strains also hybridized to the cryI-specific probe, indicating that the cryV genes are closely related to cryI genes. Two cryV-type genes, cryV1 and cryV465, were cloned from B. thuringiensis subsp. kurstaki HD-1 and B. thuringiensis subsp. entomocidus BP465, respectively, and their nucleotide sequences were determined. The CryV1 protein was toxic to Plutella xylostella and Bombyx mori, whereas the CryV465 protein was toxic only to Plutella xylostella.
摘要
分别用cryV特异性探针和cryI特异性探针进行DNA斑点杂交，以筛选24株苏云金芽孢杆菌菌株中cryV型（鳞翅目和鞘翅目特异性）以及cryI型（鳞翅目特异性）杀虫晶体蛋白基因的含量。cryV特异性探针与12株检测的苏云金芽孢杆菌菌株杂交。大多数cryV阳性菌株也与cryI特异性探针杂交，表明cryV基因与cryI基因密切相关。分别从苏云金芽孢杆菌库斯塔克亚种HD-1和苏云金芽孢杆菌昆虫亚种BP465中克隆出两个cryV型基因cryV1和cryV465，并测定了它们的核苷酸序列。CryV1蛋白对小菜蛾和家蚕有毒，而CryV465蛋白仅对小菜蛾有毒。
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