Rhee S K, Lee G M, Lee S T
Department of Biological Sciences, Korea Advanced Institute of Science and Technology, Taejun.
Appl Microbiol Biotechnol. 1996 Feb;44(6):816-22. doi: 10.1007/BF00178624.
The effect of the presence of supplementary glucose or acetate on the growth and pyridine-degrading activity of freely suspended and calcium-alginate-immobilized Pimelobacter sp. was investigated. Although the supplementary carbon sources could be degraded simultaneously with pyridine, Pimelobacter sp. exhibited a preference for pyridine over supplementary carbon sources. Thus, the pyridine-degrading activity of the freely suspended cells was not decreased significantly by the addition of either glucose (1.5-6 mM) or acetate (6-24 mM) to the pyridine (6-24 mM). In the semi-continuous immobilized cell culture, immobilized cells also exhibited a preference for pyridine over supplementary carbon sources and did not switch their substrate preference throughout the culture. Owing to a high cell concentration, the volumetric pyridine degradation rate at 24 mM pyridine in the immobilized cell culture was approximately six times higher than that in the freely suspended cell culture. Furthermore, the immobilized cells could be reused 16 times without losing their pyridine-degrading activity during the culture period tested. Taken together, the use of immobilized Pimelobacter sp. for the degradation of pyridine is quite feasible because of the preference for pyridine over supplementary carbon sources, the high volumetric pyridine degradation rate, and the reusability of immobilized cells.
研究了添加葡萄糖或乙酸盐对游离悬浮和海藻酸钙固定化的皮杆菌属细菌生长及吡啶降解活性的影响。尽管补充碳源可与吡啶同时降解，但皮杆菌属细菌对吡啶的偏好高于补充碳源。因此，向吡啶（6 - 24 mM）中添加葡萄糖（1.5 - 6 mM）或乙酸盐（6 - 24 mM）时，游离悬浮细胞的吡啶降解活性并未显著降低。在半连续固定化细胞培养中，固定化细胞对吡啶的偏好也高于补充碳源，且在整个培养过程中未改变其底物偏好。由于细胞浓度高，在固定化细胞培养中，24 mM吡啶时的体积吡啶降解率比游离悬浮细胞培养中约高六倍。此外，在测试的培养期间，固定化细胞可重复使用16次而不丧失其吡啶降解活性。综上所述，由于对吡啶的偏好高于补充碳源、体积吡啶降解率高以及固定化细胞的可重复使用性，使用固定化皮杆菌属细菌降解吡啶是相当可行的。
