Liu D, Coloe S, Pedersen J, Baird R
Melbourne Pathology, Collingwood, Vic, Australia.
FEMS Microbiol Lett. 1996 Feb 15;136(2):147-50. doi: 10.1111/j.1574-6968.1996.tb08040.x.
Dermatophytes such as Trichophyton species are common human pathogens, the infection of which results in dermatophytosis (also known as ringworm). Several laboratory tests are used routinely for the diagnosis of dermatophytosis, but they are either slow or lacking specificity. Through examination of genomic DNA from Trichophyton dermatophytes and other fungi in arbitrarily primed PCR, it was shown that a random primer 5'-ACCCGACCTG-3' produced bands of 4.3 kb, 1.9 kb, 1.7 kb and 0.7 kb in T. rubrum DNA, bands of 2.5 kb, 1.9 kb and 0.8 kb in T. mentagrophytes var. interdigitale and T. mentagrophytes var. mentagrophytes DNA, and bands of 2.5 kb, 1.9 kb, 1.5 kb and 0.9 kb in T. tonsurans DNA. This primer amplified bands of different sizes in other fungal DNA. Therefore, based on the distinct band patterns observed in arbitrarily primed PCR using this primer, T. rubrum, T. mentagrophytes and T. tonsurans dermatophytes could be rapidly differentiated.
皮肤癣菌如毛癣菌属是常见的人类病原体，其感染会导致皮肤癣菌病（也称为癣）。几种实验室检测方法常用于皮肤癣菌病的诊断，但它们要么耗时较长，要么缺乏特异性。通过在任意引物PCR中检测来自皮肤癣菌毛癣菌属和其他真菌的基因组DNA，结果显示随机引物5'-ACCCGACCTG-3'在红色毛癣菌DNA中产生了4.3 kb、1.9 kb、1.7 kb和0.7 kb的条带，在指间毛癣菌变种和须癣毛癣菌变种DNA中产生了2.5 kb、1.9 kb和0.8 kb的条带，在断发毛癣菌DNA中产生了2.5 kb、1.9 kb、1.5 kb和0.9 kb的条带。该引物在其他真菌DNA中扩增出不同大小的条带。因此，基于使用该引物的任意引物PCR中观察到的独特条带模式，可以快速区分红色毛癣菌、须癣毛癣菌和断发毛癣菌皮肤癣菌。
