Liu D, Coloe S, Baird R, Pedersen J
Melbourne Pathology, Collingwood, Victoria, Australia.
Br J Dermatol. 1997 Sep;137(3):351-5. doi: 10.1046/j.1365-2133.1997.18481941.x.
Dermatophytes are keratinophilic fungi capable of causing dermatophytosis (commonly known as tinea or ringworm) in humans and animals. Previously, we reported the differentiation of the common dermatophytes Trichophyton rubrum, T. mentagrophytes and T. tonsurans using a random primer 5'-ACCCGACCTG-3' (OPAA11) in the arbitrarily primed polymerase chain reaction (AP-PCR). In the present study, by examining additional dermatophytes including eight Microsporum spp., 16 Trichophyton species/subspecies and Epidermophyton floccosum using both OPAA11 and a second random decamer 5'-GAGAGCCAAC-3' (OPD18) in AP-PCR, we show that except for T. rubrum and T. gourvilli, and three T. mentagrophytes varieties, most of the dermatophyte fungi investigated formed distinct DNA band patterns on gel electrophoresis. The amplification of specific DNA bands in AP-PCR appeared to be independent of culture variations shown by dermatophyte isolates. These results provide the basis for the rapid identification of dermatophytes at the genetic level, supplementing existing laboratory methods and improving the diagnosis of human dermatophytosis.
皮肤癣菌是嗜角蛋白真菌，可在人和动物中引起皮肤癣菌病（通常称为癣或圆癣）。此前，我们报道了在任意引物聚合酶链反应（AP-PCR）中使用随机引物5'-ACCCGACCTG-3'（OPAA11）对常见皮肤癣菌红色毛癣菌、须癣毛癣菌和断发毛癣菌进行区分。在本研究中，通过在AP-PCR中使用OPAA11和第二个随机十聚体5'-GAGAGCCAAC-3'（OPD18）检测另外的皮肤癣菌，包括8种小孢子菌属、16种毛癣菌物种/亚种和絮状表皮癣菌，我们发现除了红色毛癣菌和古氏毛癣菌以及3个须癣毛癣菌变种外，大多数所研究的皮肤癣菌在凝胶电泳上形成了独特的DNA条带模式。AP-PCR中特定DNA条带的扩增似乎与皮肤癣菌分离株所显示的培养差异无关。这些结果为在基因水平快速鉴定皮肤癣菌提供了依据，补充了现有的实验室方法并改善了人类皮肤癣菌病的诊断。
