对VP7血清型6牛轮状病毒株NCDV、B641和UK的VP4进行氨基酸序列比较分析。
Comparative amino acid sequence analysis of VP4 for VP7 serotype 6 bovine rotavirus strains NCDV, B641, and UK.
作者信息
Hardy M E, Woode G N, Xu Z C, Gorziglia M
机构信息
Department of Veterinary Pathobiology, College of Veterinary Medicine, Texas A&M University, College Station 77843-4467.
出版信息
J Virol. 1991 Oct;65(10):5535-8. doi: 10.1128/JVI.65.10.5535-5538.1991.
In a previous study (S. Zheng, G. N. Woode, D. R. Melendy, and R. F. Ramig, J. Clin. Microbiol. 27:1939-1945, 1989), it was predicted that the VP7 serotype 6 bovine rotavirus strains NCDV and B641 do not share antigenically similar VP4s. In this study, gene 4 and the VP7 gene of B641 were sequenced, and the amino acid sequences were deduced and compared with those of NCDV and bovine rotavirus strain UK. Amino acid sequence homology in VP7 between the three strains was greater than 94%, confirming their relationship as VP7 serotype 6 viruses. VP4 of B641 showed amino acid homology to UK of 94% but only 73% homology to NCDV. Sequence comparison of a variable region of VP8 demonstrated amino acid homology of 53% between B641 and NCDV, whereas B641 and UK were 89% homologous in this region. These results confirm the earlier prediction that although the same serotype by VP7 reactivity, B641 and NCDV represent different VP4 serotypes. This difference in VP4 may have contributed to the lack of homotypic protection observed in calves, implicating VP4 as an important antigen in the active immune response to rotavirus infection in bovines.
在之前的一项研究中（S. 郑、G. N. 伍德、D. R. 梅伦迪和R. F. 拉米格，《临床微生物学杂志》27:1939 - 1945，1989年），曾预测VP7血清型6牛轮状病毒株NCDV和B641不具有抗原相似的VP4。在本研究中，对B641的基因4和VP7基因进行了测序，推导了氨基酸序列，并与NCDV和牛轮状病毒株UK的氨基酸序列进行了比较。这三种毒株在VP7中的氨基酸序列同源性大于94%，证实了它们作为VP7血清型6病毒的关系。B641的VP4与UK的氨基酸同源性为94%，但与NCDV的同源性仅为73%。VP8可变区的序列比较显示，B641和NCDV之间的氨基酸同源性为53%，而在该区域B641和UK的同源性为89%。这些结果证实了早期的预测，即尽管根据VP7反应性属于同一血清型，但B641和NCDV代表不同的VP4血清型。VP4的这种差异可能导致了在犊牛中观察到的同型保护的缺乏，这表明VP4是牛轮状病毒感染的主动免疫反应中的一种重要抗原。
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