Roggendorf W, Strupp S, Paulus W
Institute of Pathology (Neuropathology), University of Würzburg, Germany.
Acta Neuropathol. 1996 Sep;92(3):288-93. doi: 10.1007/s004010050520.
The role of inflammatory reactions in brain tumors is still unclear. In particular, there is little information about the participation of the microglia/macrophage cell system. We therefore investigated 72 surgical biopsy samples of brain tumors (astrocytoma, glioblastoma, oligodendroglioma, ependymoma, medulloblastoma, cerebral lymphoma, gangliocytoma, neurocytoma and germinoma) and the brains of eight cases with malignant gliomas that came to autopsy, using immunohistochemical markers for the monocyte/macrophage lineage (Ki-M1P, HLA-DR, KP1, My4, My7, Ki-M1, Ki-M6, EBM 11). These markers allowed us to characterize four subtypes of the microglia/macrophage cell system: ramified microglia, ameboid microglia, perivascular microglia and brain macrophages. Among the different tumors, glioblastomas and anaplastic gliomas showed the largest number of mixed cell populations, which consisted of macro-phages and ramified and ameboid microglia. In glial tumors of low malignancy fewer, predominantly ameboid, microglia were found. Neuronal tumors showed only a mild increase of microglia. Cerebral lymphomas contained macrophages diffusely distributed within the tumor center, while activated microglia were prominent at the border zone and in the adjacent brain tissue. The autopsy cases were used to study the morphometric distribution of microglia/macrophages. There was a significant increase of microglia/macrophages within the tumor, but no differences were seen between central and peripheral tumor areas. The non-neoplastic gray and white matter contained more microglial cells than controls. We conclude that the distribution pattern of ameboid and ramified microglial cells and macrophages is distinct in most of the investigated tumor types, underlining the complex immunological function of the microglia/macrophage cell system.
炎症反应在脑肿瘤中的作用仍不清楚。特别是，关于小胶质细胞/巨噬细胞系统的参与情况几乎没有相关信息。因此，我们使用单核细胞/巨噬细胞谱系的免疫组化标记物（Ki-M1P、HLA-DR、KP1、My4、My7、Ki-M1、Ki-M6、EBM 11），对72例脑肿瘤手术活检样本（星形细胞瘤、胶质母细胞瘤、少突胶质细胞瘤、室管膜瘤、髓母细胞瘤、脑淋巴瘤、神经节细胞瘤、神经细胞瘤和生殖细胞瘤）以及8例死于恶性胶质瘤的尸检大脑进行了研究。这些标记物使我们能够区分小胶质细胞/巨噬细胞系统的四种亚型：分枝状小胶质细胞、阿米巴样小胶质细胞、血管周围小胶质细胞和脑巨噬细胞。在不同的肿瘤中，胶质母细胞瘤和间变性胶质瘤显示出最多的混合细胞群，其中包括巨噬细胞以及分枝状和阿米巴样小胶质细胞。在低恶性度的胶质肿瘤中，发现的小胶质细胞较少，主要是阿米巴样小胶质细胞。神经元肿瘤仅显示小胶质细胞轻度增加。脑淋巴瘤在肿瘤中心弥漫分布有巨噬细胞，而活化的小胶质细胞在边界区和邻近脑组织中较为突出。尸检病例用于研究小胶质细胞/巨噬细胞的形态计量分布。肿瘤内小胶质细胞/巨噬细胞显著增加，但肿瘤中央和周边区域未见差异。非肿瘤性灰质和白质中的小胶质细胞比对照组更多。我们得出结论，在大多数研究的肿瘤类型中，阿米巴样和分枝状小胶质细胞以及巨噬细胞的分布模式是不同的，这突出了小胶质细胞/巨噬细胞系统复杂的免疫功能。
