Nishibori M, Nakaya N, Tahara A, Kawabata M, Mori S, Saeki K
Department of Pharmacology, Okayama University Medical School, Japan.
Neurosci Lett. 1996 Aug 9;213(3):193-6. doi: 10.1016/0304-3940(96)12864-0.
We investigated the immunohistochemical localization of a cytokine macrophage migration inhibitory factor (MIF) in the bovine brain. MIF was present in the ependymal cell linings of the cerebral ventricles throughout. Double immunostaining of the section with anti-glial fibrillary acidic protein (GFAP) antibody and with anti-MIF antibody showed that the astrocytes present in subependymal layer were immunoreactive for MIF. In the hippocampus, the pyramidal cells in the CA3 and CA4 subfields and the granule cells of the dentate gyrus were immunoreactive. The bundles of mossy fibers were stained along their projections to CA3 and CA4 regions. The nuclei of the subpopulation of these MIF-immunoreactive cells were also immunostained. These results indicated the widespread distribution of a cytokine, MIF, in the bovine brain and suggested the possibility that MIF might play additional roles than a proinflammatory mediator role in the brain.
我们研究了细胞因子巨噬细胞移动抑制因子（MIF）在牛脑中的免疫组织化学定位。MIF 始终存在于脑室的室管膜细胞内衬中。用抗胶质纤维酸性蛋白（GFAP）抗体和抗 MIF 抗体对切片进行双重免疫染色显示，室管膜下层的星形胶质细胞对 MIF 呈免疫反应性。在海马体中，CA3 和 CA4 亚区的锥体细胞以及齿状回的颗粒细胞呈免疫反应性。苔藓纤维束沿其向 CA3 和 CA4 区域的投射被染色。这些 MIF 免疫反应性细胞亚群的细胞核也被免疫染色。这些结果表明细胞因子 MIF 在牛脑中广泛分布，并提示 MIF 在脑中可能发挥除促炎介质作用之外的其他作用。
