乳腺癌患者血清对小鼠乳腺肿瘤病毒的病毒溶解作用。
Virolysis of mouse mammary tumor virus by sera from breast cancer patients.
作者信息
Witkin S S, Egeli R A, Sarkar N H, Good R A, Day N K
出版信息
Proc Natl Acad Sci U S A. 1979 Jun;76(6):2984-7. doi: 10.1073/pnas.76.6.2984.
All type C retroviruses are lysed by human serum in apparently antibody-independent, complement-mediated reactions. In contrast, we have now determined that the mouse mammary tumor virus (MMTV), a type B retrovirus, is not disrupted by normal human serum. MMTV was lysed, however, when rabbit antibody to whole MMTV was added to the serum. By taking advantage of this dependence of MMTV lysis on specific antibody, a virolytic assay was developed, based on the measurement of reverse transcriptase released from disrupted virions, to search for evidence of antibodies to MMTV in human sera. Significantly greater virolytic activity was detected in the sera of patients with breast cancer than in sera of patients with benign disease (P less than 0.001) or colorectal cancer (P less than 0.001) or in sera from apparently healthy individuals (P less than 0.002). This assay thus appears to be able to detect a unique attribute, possibly the presence of an antibody crossreacting with MMTV, in serum in patient with breast cancer.
所有C型逆转录病毒在明显不依赖抗体、由补体介导的反应中会被人血清裂解。相比之下，我们现在已经确定，B型逆转录病毒小鼠乳腺肿瘤病毒（MMTV）不会被正常人血清破坏。然而，当向血清中加入针对完整MMTV的兔抗体时，MMTV会被裂解。利用MMTV裂解对特异性抗体的这种依赖性，基于对从被破坏的病毒粒子中释放的逆转录酶的测量，开发了一种病毒裂解测定法，以寻找人血清中抗MMTV抗体的证据。在乳腺癌患者的血清中检测到的病毒裂解活性明显高于良性疾病患者（P<0.001）、结直肠癌患者（P<0.001）或明显健康个体的血清（P<0.002）。因此，该测定法似乎能够检测出乳腺癌患者血清中的一种独特属性，可能是存在与MMTV发生交叉反应的抗体。
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