Levin A, Brubaker G, Shao J S, Kumby D, O'Brien T R, Goedert J J, Strauss K W, Blattner W A, Hannet I
Department of Virology, Medical College of St Bartholomew's Hospital, London, UK.
Int J STD AIDS. 1996 Jul;7(4):288-91. doi: 10.1258/0956462961917825.
With the FACSCount flow cytometer, counts of CD4, CD8 and CD3 lymphocytes and CD4/CD8 ratios were performed in a rural hospital in Tanzania. A total of 168 subjects (21 HIV-1 seropositive and 147 HIV-1 seronegative) were tested as part of a population-based serosurvey and AIDS education programme; 134 other subjects were hospitalized patients who had signs and symptoms suggestive of AIDS (69 HIV-1 seropositive and 65 HIV-seronegative). Mean values for the 147 HIV-1 seronegative subjects from the local population were 980 CD4 cells (95% CI 930, 1031), 598 CD8 cells (560, 635) and CD4/CD8 ratio 1.78 (1.68, 1.89). Seropositive subjects from the local population had significantly lower CD4 cell counts, higher CD8 counts and a lower CD4/CD8 ratio. CD4 cells were significantly lower and CD8 cells significantly higher in HIV-1 seropositive hospital patients compared to HIV-1 seronegative patients. However, 23 (35%) seronegative hospital patients had CD4 counts lower than 600. These results establish baseline values for the lymphocyte subsets in this population and indicate that this technique can be used in remote areas to monitor progress of HIV-infected individuals.
使用FACSCount流式细胞仪，在坦桑尼亚的一家乡村医院对CD4、CD8和CD3淋巴细胞进行计数，并计算CD4/CD8比值。作为一项基于人群的血清学调查和艾滋病教育项目的一部分，共检测了168名受试者（21名HIV-1血清阳性和147名HIV-1血清阴性）；另外134名受试者为有艾滋病体征和症状的住院患者（69名HIV-1血清阳性和65名HIV血清阴性）。当地人群中147名HIV-1血清阴性受试者的平均数值为CD4细胞980个（95%可信区间930, 1031），CD8细胞598个（560, 635），CD4/CD8比值1.78（1.68, 1.89）。当地人群中的血清阳性受试者CD4细胞计数显著更低，CD8计数更高，CD4/CD8比值更低。与HIV-1血清阴性患者相比，HIV-1血清阳性住院患者的CD4细胞显著更低，CD8细胞显著更高。然而，23名（35%）血清阴性住院患者的CD4计数低于600。这些结果确立了该人群淋巴细胞亚群的基线值，并表明该技术可用于偏远地区监测HIV感染者的病情进展。
