通过低频限制性酶切位点PCR和随机扩增多态性DNA分析对少动罗尔斯通氏菌进行DNA指纹图谱分析
DNA fingerprinting of Ralstonia paucula by infrequent-restriction-site PCR and randomly amplified polymorphic DNA analysis.
作者信息
Moissenet Didier, Vu-Thien Hoang, Benzerara Yahia, Arlet Guillaume
机构信息
Service de Microbiologie, Hôpital d'enfants Armand-Trousseau AP-HP, Paris, France.
出版信息
J Clin Microbiol. 2003 Dec;41(12):5747-9. doi: 10.1128/JCM.41.12.5747-5749.2003.
Abstract
Ralstonia paucula (formerly CDC group IV c-2) is an environmental organism that can cause serious human infections, occasionally clusters of nosocomial infections. In the present work, 26 strains of R. paucula (4 from the American Centers for Disease Control and Prevention collection, 10 from the Belgian Laboratorium voor Microbiologie [LMG] collection, and 12 French clinical isolates) were analyzed with infrequent-restriction-site PCR and randomly amplified polymorphic DNA analysis. Both techniques accurately distinguished between collection strains. Two close patterns obtained for all the French isolates suggested a clonal strain. Two LMG collection strains originating from human sources in the United States also showed patterns close to those of French isolates.
摘要
少动罗尔斯通氏菌（以前的疾病控制和预防中心IV c-2组）是一种环境微生物，可引起严重的人类感染，偶尔会出现医院感染聚集。在本研究中，对26株少动罗尔斯通氏菌（4株来自美国疾病控制与预防中心菌种保藏中心，10株来自比利时微生物学实验室[LMG]菌种保藏中心，12株为法国临床分离株）进行了低频限制性位点PCR和随机扩增多态性DNA分析。两种技术都能准确区分保藏菌株。所有法国分离株获得的两种相似模式表明存在一个克隆菌株。两株源自美国人类样本的LMG菌种保藏中心菌株也显示出与法国分离株相似的模式。
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