Baltzinger M, Relaix F, Remy P
CNRS/UPR 9005, Mécanismes Moléculaires de la Division Cellulaire et du Développement, Strasbourg, France.
Mech Dev. 1996 Aug;58(1-2):103-14. doi: 10.1016/s0925-4773(96)00563-1.
XLPOU3 is a member of the Xenopus POU domain family of genes, encoding a class of homeodomain-containing transcription factors implicated in cell differentiation during development. Here we describe the isolation and characterisation of XLPOU3b, an allelic genomic counterpart of the XLPOU3 cDNA previously described (Baltzinger et al. (1992) Nucleic Acids Res. 20, 1993), and the spatio-temporal transcription patterns of this gene during development, as determined by Northern blotting and whole-mount in situ hybridisation. Like other genes encoding POU domains of class III, XLPOU3b is intronless. XLPOU3 and XLPOU3b proteins share 82% amino acid identity with the mammalian N-Oct-3/Brn-2 proteins. XLPOU3 mRNA, which is first detected at the neurula stage, is expressed in the developing brain and spinal cord. In addition, XLPOU3 transcription is observed in a restricted region of the auditory vesicle during development. The results suggest that XLPOU3 may participate in patterning the central nervous system during early Xenopus development.
XLPOU3是非洲爪蟾POU结构域基因家族的成员，编码一类在发育过程中参与细胞分化的含同源结构域的转录因子。在此，我们描述了XLPOU3b的分离与特性，它是先前所述XLPOU3 cDNA的等位基因基因组对应物（Baltzinger等人，(1992)《核酸研究》20, 1993），以及通过Northern印迹法和整体原位杂交确定的该基因在发育过程中的时空转录模式。与其他编码III类POU结构域的基因一样，XLPOU3b没有内含子。XLPOU3和XLPOU3b蛋白与哺乳动物的N-Oct-3/Brn-2蛋白有82%的氨基酸同一性。XLPOU3 mRNA在神经胚阶段首次被检测到，在发育中的脑和脊髓中表达。此外，在发育过程中，在听泡的一个受限区域观察到XLPOU3转录。结果表明，XLPOU3可能在非洲爪蟾早期发育过程中参与中枢神经系统的模式形成。
