Masuzawa T, Wilske B, Komikado T, Suzuki H, Kawabata H, Sato N, Muramatsu K, Sato N, Isogai E, Isogai H, Johnson R C, Yanagihara Y
Department of Microbiology, School of Pharmaceutical Sciences, University of Shizuoka, Japan.
Microbiol Immunol. 1996;40(8):539-45. doi: 10.1111/j.1348-0421.1996.tb01106.x.
Sixty-one Borrelia burgdorferi sensu lato strains from various sources (ticks, human, and wild animals) in Japan and two strains from ticks in Far Eastern Russia were classified on the basis of reactivity with 16 monoclonal antibodies (mAb) to outer surface protein A (OspA) and by DNA-DNA hybridization assay. Eleven OspA serotypes (J1 to J11) were recognized among the Japanese and the Far East Russian isolates (serotypes J1 to J9 were identified as B. garinii, serotype J10 was identified as B. afzelii, and serotype J11 corresponded to B. japonica), whereas 7 OspA serotypes for North American and European isolates previously reported (Bettina Wilske et al, J. Clin. Microbiol. 31:340-350,1993) were not observed except for OspA serotype 2 which showed identical reactivity with OspA serotype J10. This finding provides helpful information for understanding the geographical distribution of Lyme disease borrelia and the development of vaccine and diagnostic tests.
1.B. burgdorferi sensu stricto has not been observed in Japan, 2. Japanese B. afzelii isolates are closely related to those from Europe, 3. B. garinii isolates from Japan are highly heterogeneous and apparently different from European B. garinii isolates.
根据与16种针对外表面蛋白A（OspA）的单克隆抗体（mAb）的反应性以及DNA-DNA杂交试验，对来自日本不同来源（蜱、人类和野生动物）的61株伯氏疏螺旋体狭义种菌株以及来自俄罗斯远东地区蜱的2株菌株进行了分类。在日本和俄罗斯远东地区的分离株中识别出11种OspA血清型（J1至J11）（血清型J1至J9被鉴定为加氏疏螺旋体，血清型J10被鉴定为阿氏疏螺旋体，血清型J11对应日本疏螺旋体），而除了与OspA血清型J10显示相同反应性的OspA血清型2外，未观察到先前报道的北美和欧洲分离株的7种OspA血清型（贝蒂娜·威尔斯克等人，《临床微生物学杂志》31:340 - 350,1993）。这一发现为了解莱姆病疏螺旋体的地理分布以及疫苗和诊断测试的开发提供了有用信息。
