Kee S, Hwang K J, Oh H B, Park K S
Division of Serology, National Institute of Health, Seoul, Korea.
Microbiol Immunol. 1994;38(12):989-93. doi: 10.1111/j.1348-0421.1994.tb02157.x.
Two characteristic strains (935T, 934U) of B. burgdorferi isolated from Ixodes persulcatus and a wild rodent (Apodemus agrarius) in Korea were selected and analyzed by an immunoblot method using the monoclonal antibodies directed to different epitopes of outer surface protein A (OspA). The reactive pattern of strain 934U with these monoclonal antibodies was identical to that of strains belonging to B. afzelii and that of strain 935T was different from other isolates. Monoclonal antibody (5TEE3) which is specific to strain 935T did not react with any other Western and Japanese isolates. So, it was suggested that there exist at least two groups of B. burgdorferi in Korea. One could be classified as B. afzelii and the other is a divergent group from three known species of B. burgdorferi sensu stricto, B. garinii and B. afzelii.
从韩国全沟硬蜱和野生啮齿动物（黑线姬鼠）中分离出的伯氏疏螺旋体的两个特征菌株（935T、934U），使用针对外表面蛋白A（OspA）不同表位的单克隆抗体，通过免疫印迹法进行选择和分析。菌株934U与这些单克隆抗体的反应模式与阿氏疏螺旋体属菌株相同，而菌株935T的反应模式与其他分离株不同。对菌株935T特异的单克隆抗体（5TEE3）不与任何其他西方和日本分离株发生反应。因此，提示在韩国至少存在两组伯氏疏螺旋体。一组可归类为阿氏疏螺旋体，另一组是与狭义伯氏疏螺旋体、伽氏疏螺旋体和阿氏疏螺旋体这三个已知种不同的分支。
