人类3号染色体：40个与基因和cDNA同源的独特NotI连接克隆的高分辨率荧光原位杂交图谱。
Human chromosome 3: high-resolution fluorescence in situ hybridization mapping of 40 unique NotI linking clones homologous to genes and cDNAs.
作者信息
Protopopov A I, Gizatullin R Z, Vorobieva N V, Protopopova M V, Kiss C, Kashuba V I, Klein G, Kisselev L L, Graphodatsky A S, Zabarovsky E R
机构信息
Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk, Russia.
出版信息
Chromosome Res. 1996 Sep;4(6):443-7. doi: 10.1007/BF02265051.
Forty new NotI linking clones representing sequence tagged sites (STSs) were mapped by fluorescence in situ hybridization (FISH) to different regions of human chromosome 3 (HSA3). Clone NL1-245, containing human aminoacylase 1, was localized to 3p21.2-p21.1. Our previous localization of the CLC-2 chloride channel protein gene was refined to 3q27. Clone NL2-316 most likely contains a translocon-associated protein gamma-subunit gene and was mapped to 3q23-q24. To our knowledge, this is the first time this gene has been mapped. One NotI linking clone (NL1-229) probably contains a new protein phosphatase gene. This clone was mapped to 3p25. Five NotI linking clones probably contain human expressed sequence tags (ESTs), as they possess sequences with a high level of identity (> 90%) to cDNA clones. Other clones show 56-85% homology to known mammalian and human genes with various functions, including oncogenes and tumour-suppressor genes. These clones might represent new genes.
通过荧光原位杂交（FISH）将代表序列标签位点（STS）的40个新的NotI连接克隆定位到人类染色体3（HSA3）的不同区域。包含人氨基酰化酶1的克隆NL1 - 245定位于3p21.2 - p21.1。我们之前对CLC - 2氯通道蛋白基因的定位被精确到3q27。克隆NL2 - 316很可能包含一个转位因子相关蛋白γ亚基基因，并被定位到3q23 - q24。据我们所知，这是该基因首次被定位。一个NotI连接克隆（NL1 - 229）可能包含一个新的蛋白磷酸酶基因。该克隆被定位到3p25。五个NotI连接克隆可能包含人类表达序列标签（EST），因为它们具有与cDNA克隆高度同源（> 90%）的序列。其他克隆与具有各种功能的已知哺乳动物和人类基因显示出56 - 85%的同源性，包括癌基因和肿瘤抑制基因。这些克隆可能代表新基因。
Zotero 插件