Tertov V V, Sobenin I A, Orekhov A N
Institute of Experimental Cardiology, Cardiology Research Center, Russian Academy of Medical Sciences, Moscow, Russia.
Free Radic Res. 1996 Oct;25(4):313-9. doi: 10.3109/10715769609149054.
The sialic acid content of electronegative low density lipoprotein (LDL) and LDL isolated from human aortic intima was measured. Sialic acid level in electronegative LDL of healthy subjects was 1.7-fold lower than in native LDL. Sialic acid content in electronegative LDL of coronary atherosclerosis patients was 3-fold lower than in native LDL. Lipoproteins isolated from grossly normal human aortic intima and from fatty streaks contained 20-56% less sialic acid as compared to blood plasma LDL. A negative correlation was established between the ability of electronegative and aortic LDL to stimulate lipid accumulation in cells cultured from uninvolved human aortic intima and lipoprotein sialic acid content. The results obtained indicate that electronegative and aortic LDLs have a low sialic acid content, i.e., are desialylated lipoproteins. Considered together with the fact that all known atherogenic LDLs have similar characteristics, our findings suggest that modified LDLs are the same lipoprotein particles subjected to multiple modification.
测定了人主动脉内膜中分离出的带负电低密度脂蛋白（LDL）和LDL的唾液酸含量。健康受试者带负电LDL中的唾液酸水平比天然LDL低1.7倍。冠状动脉粥样硬化患者带负电LDL中的唾液酸含量比天然LDL低3倍。从大体正常的人主动脉内膜和脂肪条纹中分离出的脂蛋白与血浆LDL相比，唾液酸含量少20 - 56%。在带负电的LDL和主动脉LDL刺激未受累人主动脉内膜培养细胞中脂质积累的能力与脂蛋白唾液酸含量之间建立了负相关。所得结果表明，带负电的LDL和主动脉LDL唾液酸含量低，即去唾液酸化脂蛋白。结合所有已知致动脉粥样硬化LDL具有相似特征这一事实，我们的研究结果表明，修饰的LDL是经过多次修饰的相同脂蛋白颗粒。
