VanWinkle W B, Snuggs M B, Buja L M
Department of Pathology and Laboratory Medicine, University of Texas Health Science Center at Houston 77030, USA.
In Vitro Cell Dev Biol Anim. 1996 Sep;32(8):478-85. doi: 10.1007/BF02723051.
Tissue-cultured neonatal cardiomyocytes can be successfully maintained in culture on a variety of extracellular matrix components such as laminin, fibronectin, and interstitial collagens (Types I and III). In vivo, however, cardiomyocytes (as well as many other cells) exist in a highly complex extracellular matrix environment composed of, in addition to the above three components, other proteins, proteoglycans, and growth factors. We have developed a procedure for culturing cardiomyocytes on a naturally occurring complete extracellular matrix, Cardiogel. This substrate, synthesized by cardiac fibroblasts, contains laminin, fibronectin. Types I and III collagen, and proteoglycans. When compared to cardiomyocytes grown on laminin alone or fibronectin alone, Candiogel-supported cardiomyocytes adhere more rapidly after plating, exhibit spontaneous contractility earlier, undergo cytoskeletal and myofibrillar differentiation earlier, and grow larger than their counterparts. We suggest that their superior growth characteristics reflect the synergistic effect of numerous extracellular matrix components signals in Cardiogel transduced by the cardiomyocyte cytoskeletal elements.
组织培养的新生心肌细胞可以成功地在多种细胞外基质成分上进行培养，如层粘连蛋白、纤连蛋白和间质胶原（I型和III型）。然而，在体内，心肌细胞（以及许多其他细胞）存在于高度复杂的细胞外基质环境中，除了上述三种成分外，还包括其他蛋白质、蛋白聚糖和生长因子。我们已经开发出一种在天然完整细胞外基质Cardiogel上培养心肌细胞的方法。这种由心脏成纤维细胞合成的基质含有层粘连蛋白、纤连蛋白、I型和III型胶原以及蛋白聚糖。与单独在层粘连蛋白或纤连蛋白上生长的心肌细胞相比，Cardiogel支持的心肌细胞在接种后粘附更快，更早表现出自发性收缩，更早经历细胞骨架和肌原纤维分化，并且比对照细胞长得更大。我们认为，它们优越的生长特性反映了心肌细胞细胞骨架元件转导的Cardiogel中众多细胞外基质成分信号的协同作用。
