Belker A M, Sherins R J, Dennison-Lagos L
Department of Surgery, University of Louisville School of Medicine, Kentucky, USA.
J Androl. 1996 Jul-Aug;17(4):420-6.
Before patients with obstructive azoospermia undergo surgical reconstruction, testicular biopsy is required to establish that spermatogenesis is normal. To perform the corrective surgical procedure at the same time as the testicular biopsy, a touch imprint method of processing the biopsy specimen has replaced frozen section processing, which distorts the germinal cells. We report a rapid, simple method of staining a touch imprint specimen in the operating room. It requires less than 5 minutes and can be performed by the andrology laboratory technologist, operating room staff, or the surgeon, thereby avoiding the need to transport the biopsy specimen to the pathology laboratory.
在梗阻性无精子症患者进行手术重建之前，需要进行睾丸活检以确定生精功能正常。为了在进行睾丸活检的同时实施矫正手术，一种处理活检标本的触摸印片法已取代了冷冻切片法，因为冷冻切片法会使生殖细胞变形。我们报告一种在手术室对触摸印片标本进行染色的快速、简便方法。该方法所需时间不到5分钟，可由男科实验室技术人员、手术室工作人员或外科医生操作，从而无需将活检标本送到病理实验室。
