人RHAMM cDNA的特性：透明质酸结合结构域的保守性
The characterization of a human RHAMM cDNA: conservation of the hyaluronan-binding domains.
作者信息
Wang C, Entwistle J, Hou G, Li Q, Turley E A
机构信息
Department of Pediatrics and Physiology, Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Canada.
出版信息
Gene. 1996 Oct 3;174(2):299-306. doi: 10.1016/0378-1119(96)00080-7.
A full-length human RHAMM cDNA clone was isolated by a combination of screening a human breast cDNA expression library with the murine RHAMM 2 cDNA as well as 5' RACE and RT-PCR using messenger RNA from human breast cell line (MCF-10A). The full-length cDNA contained 725 aa that encoded an 84 kDa protein. Although the coding region of the human RHAMM cDNA resembles the murine RHAMM v4, it has additional unique N-terminal (489 bp) and C-terminal (33 bp) regions. Also, only 1 of 5 repeat sequences encoded in the murine cDNA are present in human cDNA. The overall homology between the overlapping region of human and mouse RHAMM v4 cDNA clone is 85%, but the HA binding motif (B[X7]B), shown to be critical for the signaling capability of this receptor, is 100% conserved.
通过用鼠源RHAMM 2 cDNA筛选人乳腺cDNA表达文库，以及使用来自人乳腺细胞系（MCF - 10A）的信使RNA进行5' RACE和RT - PCR相结合的方法，分离出了一个全长人RHAMM cDNA克隆。该全长cDNA包含725个氨基酸，编码一个84 kDa的蛋白质。虽然人RHAMM cDNA的编码区与鼠源RHAMM v4相似，但它有额外独特的N端（489 bp）和C端（33 bp）区域。此外，鼠源cDNA中编码的5个重复序列中只有1个存在于人cDNA中。人和小鼠RHAMM v4 cDNA克隆重叠区域的总体同源性为85%，但对该受体信号传导能力至关重要的HA结合基序（B[X7]B）是100%保守的。
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