Nakazono M, Hirai A
Graduate Division of Biochemical Regulation, School of Agricultural Sciences, Nagoya University, Japan.
Mol Gen Genet. 1993 Jan;236(2-3):341-6. doi: 10.1007/BF00277131.
The entire set of transferred chloroplast DNA sequences in the mitochondrial genome of rice (Oryza sativa cv. Nipponbare) was identified using clone banks that cover the chloroplast and mitochondrial genomes. The mitochondrial fragments that were homologous to chloroplast DNA were mapped and sequenced. The nucleotide sequences around the termini of integrated chloroplast sequences in the rice mtDNA revealed no common sequences or structures that might enhance the transfer of DNA. Sixteen chloroplast sequences, ranging from 32 bases to 6.8 kb in length, were found to be dispersed throughout the rice mitochondrial genome. The total length of these sequences is equal to approximately 6% (22 kb) of the rice mitochondrial genome and to 19% of the chloroplast genome. The transfer of segments of chloroplast DNA seems to have occurred at different times, both before and after the divergence of rice and maize. The mitochondrial genome appears to have been rearranged after the transfer of chloroplast sequences as a result of recombination at these sequences. The rice mitochondrial DNA contains nine intact tRNA genes and three tRNA pseudogenes derived from the chloroplast genome.
利用覆盖叶绿体和线粒体基因组的克隆文库，鉴定了水稻（日本晴）线粒体基因组中转移的整套叶绿体DNA序列。对与叶绿体DNA同源的线粒体片段进行了定位和测序。水稻线粒体DNA中整合叶绿体序列末端周围的核苷酸序列未显示出可能增强DNA转移的共同序列或结构。发现16个长度从32个碱基到6.8 kb不等的叶绿体序列分散在水稻线粒体基因组中。这些序列的总长度约等于水稻线粒体基因组的6%（22 kb），相当于叶绿体基因组的19%。叶绿体DNA片段的转移似乎发生在不同时期，在水稻和玉米分化之前和之后都有。由于这些序列的重组，线粒体基因组在叶绿体序列转移后似乎发生了重排。水稻线粒体DNA包含9个完整的tRNA基因和3个源自叶绿体基因组的tRNA假基因。
