破坏豌豆根瘤菌脂多糖核心四糖合成的突变体的遗传和化学特征分析
Genetic and chemical characterization of a mutant that disrupts synthesis of the lipopolysaccharide core tetrasaccharide in Rhizobium leguminosarum.
作者信息
Allaway D, Jeyaretnam B, Carlson R W, Poole P S
机构信息
School of Animal and Microbial Science, University of Reading, United Kingdom.
出版信息
J Bacteriol. 1996 Nov;178(21):6403-6. doi: 10.1128/jb.178.21.6403-6406.1996.
Abstract
A 2-kb region that complements the Tn5-derived lipopolysaccharide (LPS) rough mutant Rhizobium leguminosarum RU301 was sequenced. Two open reading frames (ORFs) were identified. The first ORF (lpcA) is homologous to a family of bacterial sugar transferases involved in LPS core tetrasaccharide biosynthesis. ORF2 (lpcB), in which Tn5 transposed, has no significant homology to any DNA in the GenBank-EMBL databases. Chemical characterization of LPS produced by strain RU301 demonstrated that the 3-deoxy-D-manno-2-octulosonic acid (Kdo) residue which normally attaches the core tetrasaccharide to the O chain was missing, suggesting that IpcB may encode a CMP-Kdo:LPS Kdo transferase.
摘要
对一个能互补源自Tn5的脂多糖（LPS）粗糙型突变体豆科根瘤菌RU301的2-kb区域进行了测序。鉴定出两个开放阅读框（ORF）。第一个ORF（lpcA）与参与LPS核心四糖生物合成的一类细菌糖基转移酶同源。ORF2（lpcB）是Tn5转座的位置，与GenBank-EMBL数据库中的任何DNA均无显著同源性。对菌株RU301产生的LPS进行化学表征表明，通常将核心四糖连接到O链上的3-脱氧-D-甘露糖-2-辛酮糖酸（Kdo）残基缺失，这表明IpcB可能编码一种CMP-Kdo:LPS Kdo转移酶。
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