感染猫免疫缺陷病毒的猫外周血淋巴细胞中凋亡的检测。

Detection of apoptosis induced in peripheral blood lymphocytes from cats infected with feline immunodeficiency virus.

作者信息

Momoi Y, Mizuno T, Nishimura Y, Endo Y, Ohno K, Watari T, Goitsuka R, Tsujimoto H, Hasegawa A

机构信息

Department of Veterinary Internal Medicine, Faculty of Agriculture, University of Tokyo, Japan.

出版信息

Arch Virol. 1996;141(9):1651-9. doi: 10.1007/BF01718289.

DOI:10.1007/BF01718289

PMID:8893788

Abstract

Peripheral blood lymphocytes (PBL) from cats infected with feline immunodeficiency virus (FIV) were examined for the occurrence of apoptosis after short-term culture. In the PBL from FIV-infected cats, changes in flow-cytometry scattergram, morphological characteristics of apoptosis and nucleosomal DNA fragmentation were observed. Percentages of apoptotic cells by flowcytometry analysis in PBL from FIV-infected cats (22.4% +/- 9.4%) were significantly higher than those in PBL from uninfected control cats (9.2% +/- 3.5%). The lymphocytes which underwent apoptosis included CD5+, CD4+ and sIgM+ cells, indicating that induction of apoptosis was not restricted to a special subset of lymphocytes. These findings provide evidence of the apoptotic state of PBL in cats with FIV infection.

摘要

对感染猫免疫缺陷病毒（FIV）的猫的外周血淋巴细胞（PBL）进行短期培养后，检测其凋亡情况。在感染FIV的猫的PBL中，观察到流式细胞仪散射图的变化、凋亡的形态学特征和核小体DNA片段化。通过流式细胞术分析，感染FIV的猫的PBL中凋亡细胞的百分比（22.4%±9.4%）显著高于未感染对照猫的PBL（9.2%±3.5%）。发生凋亡的淋巴细胞包括CD5 +、CD4 +和sIgM +细胞，这表明凋亡的诱导并不局限于淋巴细胞的特定亚群。这些发现为FIV感染猫的PBL的凋亡状态提供了证据。