用合成肽抗体检测犬细小病毒抗原

Detection of canine parvovirus antigens with antibodies to synthetic peptides.

作者信息

Vihinen-Ranta M, Lindfors E, Heiska L, Veijalainen P, Vuento M

机构信息

Department of Biological and Environmental Science, University of Jyväskylä, Finland.

出版信息

Arch Virol. 1996;141(9):1741-8. doi: 10.1007/BF01718296.

DOI:10.1007/BF01718296

PMID:8893795

Abstract

Antibodies produced in rabbits against an 18-amino acid peptide (peptide 1, NSLPQSEGATNFGDIGVP) of capsid protein VP2/residues 292-309 of canine parvovirus (CPV) or against an 18-amino acid peptide (peptide 2, GKRNTVLFHGPASTKGKS) of nonstructural protein NS1/residues 391-409 of CPV identified, in immunofluorescence analysis, viral antigens in canine A 72 cells infected with CPV. Antibodies to peptide 2 also identified viral antigens in bovine cells infected with bovine parvovirus. In western blot analysis, antibodies to peptide 1 and peptide 2 also detected viral antigens derived from blue fox parvovirus, feline parvovirus, mink enteritis virus and raccoon dog parvovirus. The peptide antibodies could be used as convenient tools in diagnosis of infections caused by CPV or closely related viruses affecting cats, minks, blue foxes and raccoon dogs.

摘要

用犬细小病毒（CPV）衣壳蛋白VP2的18个氨基酸肽段（肽段1，NSLPQSEGATNFGDIGVP）/CPV的292 - 309位残基或非结构蛋白NS1的18个氨基酸肽段（肽段2，GKRNTVLFHGPASTKGKS）/CPV的391 - 409位残基在兔体内产生的抗体，在免疫荧光分析中鉴定出感染CPV的犬A72细胞中的病毒抗原。肽段2的抗体还鉴定出感染牛细小病毒的牛细胞中的病毒抗原。在蛋白质印迹分析中，肽段1和肽段2的抗体也检测到源自蓝狐细小病毒、猫细小病毒、水貂肠炎病毒和貉细小病毒的病毒抗原。这些肽抗体可作为诊断由CPV或影响猫、水貂、蓝狐和貉的密切相关病毒引起的感染的便捷工具。