Sébire G, Delfraissy J F, Demotes-Mainard J, Oteifeh A, Emilie D, Tardieu M
Laboratoire Virus, Neurone et Immunité, UFR Kremlin-Bicêtre, Université Paris XI, France.
Cytokine. 1996 Aug;8(8):636-41. doi: 10.1006/cyto.1996.0085.
The effect of interleukin 4 (IL-4) and IL-13 on IL-6 and IL-1 beta production by human embryonic microglial cells and human peripheral blood monocyte cells (PBMC) was compared. IL-4 or IL-13 increase intra-cellular IL-6 mRNA and IL-6 titres in supernatants of microglial cells whereas they decreased IL-6 production by PBMC tested in the same conditions. IL-4 and IL-13 increased also IL-1 beta production by human microglial cells. Stimulations associating IL-4 or IL-13 and IL-1 beta had an additive effect on IL-6 production by microglial cells, whereas, in the same conditions, an inhibitory effect was observed in PBMC. In contrast, dexamethasone downregulated IL-6 production by microglial cells and PBMC. Finally, IL-1 receptor (IL-1r) type 1 and IL-1r type 2 were detected on human microglial cells but it was demonstrated that IL-13 and IL-4 acted as IL-6 inducers in human microglial cells independently of the IL-1/IL-1r pathway.
比较了白细胞介素4（IL-4）和IL-13对人胚胎小胶质细胞和人外周血单核细胞（PBMC）产生IL-6和IL-1β的影响。IL-4或IL-13可增加小胶质细胞上清液中细胞内IL-6 mRNA和IL-6滴度，而在相同条件下检测时，它们会降低PBMC产生的IL-6。IL-4和IL-13也增加了人小胶质细胞产生的IL-1β。将IL-4或IL-13与IL-1β联合刺激对小胶质细胞产生IL-6有相加作用，而在相同条件下，在PBMC中观察到抑制作用。相比之下，地塞米松可下调小胶质细胞和PBMC产生的IL-6。最后，在人小胶质细胞上检测到1型IL-1受体（IL-1r）和2型IL-1r，但已证明IL-13和IL-4在人小胶质细胞中作为IL-6诱导剂起作用，独立于IL-1/IL-1r途径。
