Bolacchi F, Marini M, Urbani A, Bongiorno L, Roda L G
Dipartimento di Medicina Sperimentale, Università degli Studi di Roma Tor Vergata, Italy.
Neurochem Res. 1996 Aug;21(8):875-84. doi: 10.1007/BF02532335.
The hydrolysis of leucine enkephalin by the proteolytic enzymes present in human and rabbit plasma has been studied by kinetic and chromatographic techniques. Data obtained indicate the existence of noticeable intraspecific differences in the kinetics of leu-enkephalin degradation, and of formation of its hydrolysis by-products. The separation of the enzymes active on the substrate and of the inhibitors active on these enzymes evidences the existence of a species specific distribution of both groups of substances. Yet, the dissimilar kinetics of the substrate hydrolysis and of formation of its hydrolysis by-products appear to arise more from diversities in the competition between the enzymes present in plasma and in the role of inhibitors than from the differences in the enkephalin-degrading enzymes. It is suggested that differences observed may be related to the existence of species specific populations of the information-carrying plasma peptides.
已通过动力学和色谱技术研究了人血浆和兔血浆中存在的蛋白水解酶对亮氨酸脑啡肽的水解作用。所获得的数据表明，亮氨酸脑啡肽降解动力学及其水解产物形成过程中存在明显的种内差异。对作用于底物的酶和作用于这些酶的抑制剂进行分离，证明了这两类物质存在物种特异性分布。然而，底物水解及其水解产物形成的不同动力学似乎更多地源于血浆中存在的酶之间竞争的差异以及抑制剂的作用，而非脑啡肽降解酶的差异。有人提出，观察到的差异可能与携带信息的血浆肽存在物种特异性群体有关。
