Babst R, Bongiorno L, Marini M, Marzano M, Spagnoli G, Roda L G, Urbani A
Surgical Research Laboratory, University of Basel, Switzerland.
Peptides. 1998;19(7):1155-63. doi: 10.1016/s0196-9781(98)00061-8.
Possible age-induced variations of the hydrolysis of leucine enkephalin in the presence of plasma enzymes were studied by kinetic and chromatographic techniques in a group of elderly individuals. Results obtained indicate that in elderly individuals the activity of enkephalin-degrading plasma enzymes is greater than in the controls; ANOVA analysis of these data indicates that the dependency of the variation of hydrolysis upon the two age groups is statistically significant. Increased substrate hydrolysis, and a modified hydrolysis pattern, appear to be associated with increased activity of the enzymes involved, and with different distribution of the individual enzymes within each class, as well as with severely reduced activity of the low molecular weight plasma inhibitors. The combination of these factors defines a characteristic hydrolysis pattern for the elderly individuals, different from that found in the controls.
采用动力学和色谱技术，对一组老年人在血浆酶存在的情况下亮氨酸脑啡肽水解可能出现的年龄诱导变化进行了研究。所得结果表明，老年人中脑啡肽降解血浆酶的活性高于对照组；对这些数据进行方差分析表明，水解变化对两个年龄组的依赖性具有统计学意义。底物水解增加以及水解模式改变，似乎与相关酶活性增加、各类别中单个酶的不同分布以及低分子量血浆抑制剂活性严重降低有关。这些因素的综合作用定义了老年人特有的水解模式，与对照组不同。
