Bongiorno L, Fuso L, Marini M, Marzano M, Nardecchia B, Roda L G, Rossi P, Urbani A
Dipartimento di Medicina Sperimentale, Università degli Studi di Roma Tor Vergata, Rome, Italy.
Immunopharmacology. 1998 May;39(2):93-105. doi: 10.1016/s0162-3109(98)00009-5.
The enzyme hydrolysis of labelled leu-enkephalin in the presence of plasma enzymes was studied by kinetic and chromatographic techniques in a group of allergopathic patients in the acute and quiescent stage; data obtained have been compared with those obtained with normal controls. Results shown indicate that in the quiescent stage substrate degradation is reduced, and that the pattern of the hydrolysis by-products is modified with respect to the controls. In the acute as compared to the quiescent stage, enkephalin hydrolysis is further reduced, and the pattern of hydrolysis by-products is further modified. ANOVA analysis of these data indicates that the dependency of hydrolysis reduction upon the grouping of subjects (i.e., controls, quiescent and acute stage) is statistically very significant. Reduced substrate hydrolysis, and modified hydrolysis pattern, appears to be associated with decreased activity of the enzymes involved and more significantly with increased activity of the low molecular weight plasma inhibitors. The combination of these two factors appears to define a hydrolysis pattern characteristic of the allergopathic subjects, similar in the quiescent and acute phase, and different from that observed in the controls.
通过动力学和色谱技术，对一组处于急性期和静止期的过敏症患者在血浆酶存在的情况下标记亮氨酸脑啡肽的酶水解进行了研究；将获得的数据与正常对照组的数据进行了比较。所示结果表明，在静止期底物降解减少，并且水解副产物的模式相对于对照组有所改变。与静止期相比，在急性期脑啡肽水解进一步减少，并且水解副产物的模式进一步改变。对这些数据的方差分析表明，水解减少对受试者分组（即对照组、静止期和急性期）的依赖性在统计学上非常显著。底物水解减少和水解模式改变似乎与所涉及酶的活性降低有关，更显著的是与低分子量血浆抑制剂的活性增加有关。这两个因素的组合似乎定义了过敏症患者特有的水解模式，在静止期和急性期相似，与对照组观察到的不同。
