Harrington W J, Bagasra O, Sosa C E, Bobroski L E, Baum M, Wen X L, Cabral L, Byrne G E, Pomerantz R J, Wood C
Department of Medicine, University of Miami School of Medicine, Florida, USA.
J Infect Dis. 1996 Nov;174(5):1101-5. doi: 10.1093/infdis/174.5.1101.
Human herpesvirus (HHV) type 8 has been detected in both classical and AIDS-related Kaposi's sarcoma, body-cavity lymphomas, and other types of tumors. HHV-8 has also been detected in DNA from peripheral blood mononuclear cells (PBMC) of some Kaposi's sarcoma patients and more readily in B cell fractions derived from panned cell subpopulations. Two patients were followed using several methods; in situ hybridization, solution-based polymerase chain reaction (PCR), and in situ PCR. HHV-8 was intermittently detected in plasma, and detection correlated with detection in PBMC. In situ PCR demonstrated HHV-8 sequences in both peripheral blood B lymphocytes and, to a lesser extent, T lymphocytes. HHV-8 may undergo periods of viremia while at other times it is undetectable and infects circulating B cells and some T cells.
在经典型和艾滋病相关的卡波西肉瘤、体腔淋巴瘤及其他类型肿瘤中均检测到了8型人类疱疹病毒（HHV）。在一些卡波西肉瘤患者外周血单个核细胞（PBMC）的DNA中也检测到了HHV-8，且在源自淘选细胞亚群的B细胞组分中更容易检测到。使用几种方法对两名患者进行了跟踪；原位杂交、基于溶液的聚合酶链反应（PCR）和原位PCR。在血浆中间歇性检测到HHV-8，且该检测结果与PBMC中的检测结果相关。原位PCR在外周血B淋巴细胞以及程度较轻的T淋巴细胞中均显示出HHV-8序列。HHV-8可能会经历病毒血症期，而在其他时候则无法检测到，并且会感染循环B细胞和一些T细胞。
