Orenstein J M, Alkan S, Blauvelt A, Jeang K T, Weinstein M D, Ganem D, Herndier B
Department of Pathology, George Washington University, Washington, DC, USA.
AIDS. 1997 Apr;11(5):F35-45. doi: 10.1097/00002030-199705000-00001.
Human herpesvirus type 8 (HHV-8) has been associated with Kaposi's sarcoma, body cavity-based lymphoma (BCBL), and multicentric Castleman's disease through DNA, in situ hybridization, and serologic studies. HHV-8 has been visualized only in HHV-8-positive/Epstein-Barr virus (EBV)-negative/ cytomegalovirus (CMV)-negative BCBL cell lines, but not in HHV-8-positive/EBV-negative/ CMV-negative Kaposi's sarcoma lesions.
Kaposi's sarcoma of the skin, lymph node, and spleen from three patients with AIDS were analysed for HHV-8, EBV and CMV DNA by polymerase chain reaction (PCR), for HHV-8 RNA (Tl.1 riboprobe) by in situ hybridization (ISH), for viral inclusions by light microscopy, and for herpesviruses by transmission electron microscopy (TEM). Sections were also labeled with Tl.1 counterstained with CD34, an endothelial cell marker.
The skin lesion was DNA PCR-positive for HHV-8 and CMV (nested, but not single PCR), the lymph node was positive for HHV-8 and EBV, and the spleen was positive for only HHV-8. TEM revealed infection by a virus displaying the typical morphology and cytopathicity of herpesviruses. Hexagonal nucleocapsids and mature enveloped virions were present in vasoformative spindle cells and mononuclear cells, often resembling lymphocytes. Extrapolating from TEM to standard light microscopy on hematoxylin and eosin-stained paraffin sections, eosinophilic, targetoid intranuclear inclusions were identified within spindle cells which often lined vascular lumina. The Tl.1-riboprobe labeled CD34+ spindle cells containing intranuclear inclusions, as well as mononuclear cells within Kaposi's sarcoma and residual lymphoid tissue.
The herpesvirus visualized in Kaposi's sarcoma lesions has morphologic and cytopathic features typical of human herpesviruses, productively infects vasoformative spindle cells and mononuclear cells, and is consistent with HHV-8. It can also form intranuclear inclusions that are identifiable by light microscopy in hematoxylin and eosin sections and by ISH.
通过DNA、原位杂交和血清学研究，人们发现8型人类疱疹病毒（HHV-8）与卡波西肉瘤、体腔淋巴瘤（BCBL）和多中心Castleman病有关。HHV-8仅在HHV-8阳性/爱泼斯坦-巴尔病毒（EBV）阴性/巨细胞病毒（CMV）阴性的BCBL细胞系中可见，而在HHV-8阳性/EBV阴性/CMV阴性的卡波西肉瘤病变中未见。
采用聚合酶链反应（PCR）分析3例艾滋病患者皮肤、淋巴结和脾脏卡波西肉瘤中的HHV-8、EBV和CMV DNA，采用原位杂交（ISH）分析HHV-8 RNA（Tl.1核糖探针），通过光学显微镜观察病毒包涵体，通过透射电子显微镜（TEM）观察疱疹病毒。切片还用Tl.1标记，并用内皮细胞标志物CD34进行复染。
皮肤病变的HHV-8和CMV DNA PCR呈阳性（巢式，但非单重PCR），淋巴结的HHV-8和EBV呈阳性，脾脏仅HHV-8呈阳性。TEM显示一种病毒感染，该病毒具有疱疹病毒典型的形态和细胞病变特征。六边形核衣壳和成熟的包膜病毒粒子存在于血管形成性梭形细胞和单核细胞中，常类似于淋巴细胞。从TEM推断到苏木精和伊红染色石蜡切片的标准光学显微镜观察，在通常排列在血管腔的梭形细胞内发现嗜酸性、靶样核内包涵体。Tl.1核糖探针标记了含有核内包涵体的CD34+梭形细胞，以及卡波西肉瘤内的单核细胞和残留淋巴组织。
在卡波西肉瘤病变中可见的疱疹病毒具有人类疱疹病毒典型的形态和细胞病变特征，能有效感染血管形成性梭形细胞和单核细胞，与HHV-8一致。它还能形成核内包涵体，在苏木精和伊红切片中通过光学显微镜和ISH均可识别。
