Osuský R, Walker S M, Ryan S J
Department of Ophthalmology, School of Medicine, University of Southern California, Los Angeles, USA.
Graefes Arch Clin Exp Ophthalmol. 1996 Oct;234(10):637-42. doi: 10.1007/BF00185298.
Macrophages play an important role in several ocular diseases. Because macrophages localized in ocular tissues may be derived from blood monocytes, the effect of vitreous [containing transforming growth factor-beta 2 (TGF-beta 2) and hyaluronic acid] on blood monocytes, maturating in the tissue to macrophages, was determined.
Human monocytes were cultured with and without vitreous in RPMI 1640 medium containing human AB serum. As a parameter of activation the release of interleukin-6 was measured by the B9 bioassay; as an indication of maturation, the content of acid phosphatase and the increase in cell size were assessed.
Monocytes in vitreous-containing medium grew more slowly than did control monocytes. Monocytes cultured in 10% vitreous released 51% less, and in 20% vitreous 73% less, interleukin-6 than control monocytes. Vitreous at 20% significantly (P = 0.0075) reduced the amount of acid phosphatase by 80% over a 4-day culture period. This reduction was partially eliminated with neutralizing antibodies to TGF-beta (P = 0.0014). Furthermore, human recombinant TGF-beta 2 increased the activity of acid phosphatase in monocytes at 1.25 ng/ml and reduced it (P < 0.0001) at higher concentrations (5-10 ng/ml). Hyaluronic acid showed an effect additive to that of TGF-beta in further diminishing the amount of acid phosphatase (P = 0.026).
Vitreous exerts a regulatory effect on monocyte activation and maturation by its content of TGF-beta and possibly hyaluronic acid and may, thus, modify the inflammatory or immune response in the eye.
巨噬细胞在多种眼部疾病中发挥重要作用。由于眼部组织中的巨噬细胞可能来源于血液中的单核细胞，因此我们研究了玻璃体（含有转化生长因子-β2 （TGF-β2） 和透明质酸）对在组织中成熟为巨噬细胞的血液单核细胞的影响。
将人类单核细胞在含有人类AB血清的RPMI 1640培养基中分别在有或无玻璃体的情况下进行培养。通过B9生物测定法测量白细胞介素-6的释放作为激活参数；通过评估酸性磷酸酶的含量和细胞大小的增加作为成熟的指标。
含有玻璃体的培养基中的单核细胞生长比对照单核细胞慢。在10%玻璃体中培养的单核细胞释放的白细胞介素-6比对照单核细胞少51%，在20%玻璃体中培养的单核细胞释放的白细胞介素-6比对照单核细胞少73%。在为期4天的培养期内，20%的玻璃体显著（P = 0.0075）使酸性磷酸酶的量减少了80%。用TGF-β中和抗体可部分消除这种减少（P = 0.0014）。此外，人重组TGF-β2在1.25 ng/ml时增加单核细胞中酸性磷酸酶的活性，而在较高浓度（5 - 10 ng/ml）时降低其活性（P < 0.0001）。透明质酸在进一步减少酸性磷酸酶量方面显示出与TGF-β相加的作用（P = 0.026）。
玻璃体通过其所含的TGF-β以及可能的透明质酸对单核细胞的激活和成熟发挥调节作用，从而可能改变眼部的炎症或免疫反应。
