使用引物原位标记技术（PRINS）在人类配子、受精卵和植入前胚胎中快速检测染色体。
Rapid chromosome detection in human gametes, zygotes, and preimplantation embryos using the PRINS technique.
作者信息
Pellestor F, Girardet A, Lefort G, Andréo B, Charlieu J P
机构信息
CNRS UPR 9008, Montpellier, France.
出版信息
J Assist Reprod Genet. 1996 Sep;13(8):675-80. doi: 10.1007/BF02069648.
PURPOSE
The analysis of the chromosomal constitution of human gametes and embryos is of particular importance for investigation of aneuploidy occurrence and diagnostic purposes. The PRINS method constitutes an alternative to FISH for in situ chromosomal identification. We have adapted this method to human gametes, zygotes, and preimplantation embryos.
RESULTS
Chromosome-specific labeling was obtained in gametes, zygotes, and isolated blastomeres. Simultaneous detection of two or three chromosomes can be completed in less than 3 hr using fluorochrome-labeled nucleotides.
CONCLUSIONS
The PRINS technique appears to be more efficient than FISH for detection and discrimination of alpha-satellite DNA sequences. The present study demonstrates the usefulness of PRINS for chromosomal screening and preimplantation diagnosis.
目的
分析人类配子和胚胎的染色体组成对于非整倍体发生的研究及诊断目的具有特别重要的意义。引物原位标记法（PRINS）是用于原位染色体鉴定的一种替代荧光原位杂交（FISH）的方法。我们已将此方法应用于人类配子、受精卵及植入前胚胎。
结果
在配子、受精卵及分离的卵裂球中获得了染色体特异性标记。使用荧光染料标记的核苷酸，可在不到3小时内完成对两条或三条染色体的同时检测。
结论
在检测和区分α-卫星DNA序列方面，引物原位标记技术似乎比荧光原位杂交更有效。本研究证明了引物原位标记法在染色体筛查和植入前诊断中的实用性。
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