利用抗鞭毛蛋白单克隆抗体对大肠杆菌O157及其他血清型的非运动性变体进行鉴定
Characterization of nonmotile variants of Escherichia coli O157 and other serotypes by using an antiflagellin monoclonal antibody.
作者信息
Feng P, Fields P I, Swaminathan B, Whittam T S
机构信息
Division of Microbiological Studies, Food and Drug Administration, Washington, D.C. 20204, USA.
出版信息
J Clin Microbiol. 1996 Nov;34(11):2856-9. doi: 10.1128/jcm.34.11.2856-2859.1996.
Abstract
An antiflagellin monoclonal antibody (15D8) was used to detect the presence of flagella in nonmotile variants of several pathogenic Escherichia coli serotypes. Of the 48 isolates examined, 15 reacted with monoclonal antibody 15D8 and were culturally confirmed to be motile. Of the 38 clinical strains designated O157:NM or O157:H-, 7 were antibody reactive and motile and agglutinated with anti-H7 sera.
摘要
一种抗鞭毛蛋白单克隆抗体（15D8）被用于检测几种致病性大肠杆菌血清型的无运动性变体中鞭毛的存在。在所检测的48株分离株中，有15株与单克隆抗体15D8发生反应，经培养确认具有运动性。在指定为O157:NM或O157:H-的38株临床菌株中，有7株具有抗体反应性且能运动，并与抗H7血清发生凝集反应。
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